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Structural basis for impaired oxygen evolution in extrinsic-protein-reconstituted photosystem II

Authors: Nakajima, Y., Kato, K., Shen, J.-R., Nagao, R.

Date: 2026-01-12 · Version: 1
DOI: 10.64898/2026.01.11.698909

Category: Plant Biology

Model Organism: Thermosynechococcus vulcanus

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The study presents a 2.0 Å X‑ray structure of a PsbO/V/U‑reconstituted Photosystem II from the cyanobacterium Thermosynechococcus vulcanus, showing that the overall Mn₄CaO₅ cluster geometry is maintained but with subtle bond changes and loss of key water molecules in the O1 channel. These structural perturbations, including altered bicarbonate interactions and disrupted hydrogen‑bond networks for water delivery, likely explain the reduced oxygen‑evolving activity of the reconstituted complex.

Photosystem II Thermosynechococcus vulcanus PsbO PsbV PsbU