High-frequency sorghum transformation toolkit enhances Cas9 efficiency and expands promoter-editing capability with SpRY
Authors: Shen, J., Aregawi, K., Anwar, S., Miller, T., Groover, E. D., Rajkumar, M., Savage, D. F., Lemaux, P. G.
Date: 2025-12-07 · Version: 2
DOI: 10.1101/2025.01.21.634149 Category: Plant Biology
Model Organism: Sorghum bicolor
▶ AI Summary
The study presents an optimized Agrobacterium-mediated transformation toolkit for Sorghum bicolor that achieves up to 95.7% editing efficiency using CRISPR/Cas9 targeting the SbPDS gene, and demonstrates comparable performance with a PAM‑broadened SpRY variant. This platform enables multiplex genome editing and is positioned for integration of advanced tools such as prime and base editors to accelerate sorghum breeding.
Sorghum bicolor CRISPR/Cas9 Agrobacterium-mediated transformation SpRY (PAM‑flexible Cas9) high-efficiency genome editing