Genetius

The study characterizes the single-copy S-nitrosoglutathione reductase 1 (MpGSNOR1) in the liverwort Marchantia polymorpha, showing that loss-of-function mutants generated via CRISPR/Cas9 exhibit marked morphological defects and compromised SNO homeostasis and immune responses. These findings indicate that GSNOR-mediated regulation of S‑nitrosylation is an ancient mechanism linking development and immunity in early land plants.

Phylogenetic analysis reveals that non‑seed plants, exemplified by the liverwort Marchantia polymorpha, possess a streamlined repertoire of cyclin and CDK genes, with only three cyclins active in a phase‑specific manner during vegetative development. Single‑cell RNA‑seq and fluorescent reporter assays, combined with functional overexpression studies, demonstrate the distinct, non‑redundant roles of MpCYCD;1, MpCYCA, and MpCYCB;1 in G1 entry, S‑phase progression, and G2/M transition, respectively.