Genetius - Plant Biology Papers

Latest 2 Papers

Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta

Authors: Abate, B. A., Hahn, F., Chirivi, D., Betti, C., Fornara, F., Molloy, J. C., Krainer, K. M. C.

Date: 2025-11-09 · Version: 1

DOI: 10.1101/2025.11.09.687425

Category: Plant Biology

▶ AI Summary

The authors introduce the ENABLE(R) Gene Editing in planta toolkit, a streamlined two‑step cloning system for creating CRISPR/Cas9 knockout vectors suitable for transient or stable transformation. Validation was performed in Oryza sativa protoplasts and Arabidopsis thaliana plants, and the toolkit includes low‑cost protocols aimed at facilitating adoption in the Global South.

CRISPR/Cas9 plant gene editing low‑cost cloning Global South agriculture ENABLE(R) toolkit

Large-scale single-cell profiling of stem cells uncovers redundant regulators of shoot development and yield trait variation

Authors: Xu, X., Passalacqua, M., Rice, B., Demesa-Arevalo, E., Kojima, M., Takebayashi, Y., Harris, B., Sakakibara, H., Gallavotti, A., Gillis, J., Jackson, D.

Date: 2025-04-17 · Version: 2

DOI: 10.1101/2024.03.04.583414

Category: Plant Biology

Model Organism: Multi-species

▶ AI Summary

The study finely dissected shoot stem cell–enriched tissues from maize and Arabidopsis thaliana and optimized single‑cell RNA‑seq protocols to reliably capture CLAVATA3 and WUSCHEL‑expressing cells. Cross‑species comparison and functional validation, including spatial transcriptomics and mutant analyses, revealed conserved ribosome‑associated RNA‑binding proteins and sugar‑kinase families as key regulators linked to shoot development and yield traits.

single-cell RNA sequencing shoot stem cells Arabidopsis thaliana Zea mays stem cell regulators