Golden Promise-rapid, a fast-cycling barley genotype with high transformation efficiency
Authors: Buchmann, G., Haraldsson, E. B., Schüller, R., Rütjes, T., Walla, A. A., von Korff Schmising, M., Liu, S.
Date: 2025-10-31 · Version: 1
DOI: 10.1101/2025.10.31.685778 Category: Plant Biology
Model Organism: Hordeum vulgare
▶ AI Summary
The authors created a fast‑cycling, isogenic barley line (GP‑rapid) by introgressing the wild‑type Ppd‑H1 allele from Igri into the Golden Promise cultivar and performing two backcrosses to limit the donor genome, achieving a 25% reduction in generation time under speed‑breeding conditions while retaining high transformation efficiency. CRISPR/Cas9‑mediated editing of Ppd‑H1 showed regeneration and transformation rates comparable to the original Golden Promise, establishing GP‑rapid as a rapid platform for transgenic and gene‑edited barley research.
Golden Promise Ppd-H1 speed breeding CRISPR/Cas9 transformation efficiency