Developing a Molecular Toolkit to ENABLE all to apply CRISPR/Cas9-based Gene Editing in planta
Authors: Abate, B. A., Hahn, F., Chirivi, D., Betti, C., Fornara, F., Molloy, J. C., Krainer, K. M. C.
Date: 2025-11-09 · Version: 1
DOI: 10.1101/2025.11.09.687425 Category: Plant Biology
Model Organism: Multi-species
▶ AI Summary
The authors introduce the ENABLE(R) Gene Editing in planta toolkit, a streamlined two‑step cloning system for creating CRISPR/Cas9 knockout vectors suitable for transient or stable transformation. Validation was performed in Oryza sativa protoplasts and Arabidopsis thaliana plants, and the toolkit includes low‑cost protocols aimed at facilitating adoption in the Global South.
CRISPR/Cas9 plant gene editing low‑cost cloning Global South agriculture ENABLE(R) toolkit