Choosing the Best Route: Comparative Optimization of Wheat Transformation Methods for Improving Yield by Targeting TaARE1-D with CRISPR/Cas9
Authors: Tek, M. I., Budak Tek, K., Sarikaya, P., Ahmed, A. R., Fidan, H.
Date: 2025-09-12 · Version: 1
DOI: 10.1101/2025.09.11.675438 Category: Plant Biology
Model Organism: Triticum aestivum
▶ AI Summary
The researchers optimized Agrobacterium‑mediated transformation parameters for immature embryo, callus, and in‑planta methods in wheat, achieving efficiencies of up to 66.84% and shortening the callus induction stage by about one month. Validation with CRISPR/Cas9 knockout of the negative regulator TaARE1‑D produced mutants showing increased grain number, spike length, grain size, thousand‑grain weight, and a stay‑green phenotype, demonstrating the protocol’s potential to accelerate yield‑enhancing gene editing.
wheat CRISPR/Cas9 transformation optimization Agrobacterium yield traits