Arabidopsis REM transcription factors and GDE1 shape the DNA methylation landscape through the recruitment of RNA Polymerase IV transcription complexes.
Authors: Wu, Z., Xue, Y., Wang, S., Shih, Y.-H., Zhong, Z., Feng, S., Draper, J., Lu, A., Sha, J., Li, L., Wohlschlegel, J., Wu, K., Jacobsen, S. E.
Date: 2025-02-23 · Version: 1
DOI: 10.1101/2025.02.21.639493 Category: Plant Biology
Model Organism: Arabidopsis thaliana
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The study identifies four Arabidopsis REM transcription factors (VDD, VAL, REM12, REM13) that bind specific DNA sequences and, together with GDE1, recruit RNA polymerase IV to produce 24‑nt siRNAs that direct DNA methylation at designated loci. Loss of GDE1 causes Pol IV complexes to relocalize to sites bound by REM8, indicating that REM proteins provide sequence‑specific cues for epigenetic patterning.
DNA methylation 24‑nt siRNA REM transcription factors RNA polymerase IV Arabidopsis