A comprehensive multi‑environment trial of 437 maize testcross hybrids derived from 38 MLN‑tolerant lines and 29 testers identified additive genetic effects as the primary driver of grain yield, disease resistance, and drought tolerance. Strong general combining ability and specific combining ability patterns were uncovered, with top hybrids delivering up to 5.75 t ha⁻¹ under MLN pressure while maintaining high performance under optimum and drought conditions. The study provides a framework for selecting elite parents and exploiting both additive and non‑additive effects to develop resilient maize hybrids for sub‑Saharan Africa.
Nanoclustering of a plant transcription factor enables strong yet specific DNA binding
Authors: Arfman, K., Janssen, B. P. J., Romein, R., van den Boom, S., van der Woude, M., Jansen, L., Rademaker, M., Hernandez-Garcia, J., Ramalho, J. J., Dipp-Alvarez, M., Borst, J. W., Weijers, D., van Mierlo, C. P. M., Sprakel, J.
The study reveals that the Auxin Response Factor MpARF2 in Marchantia polymorpha forms nanoscopic clusters within the plant nucleus, representing a distinct mode of DNA binding distinct from monomeric/oligomeric binding and liquid phase-separated condensates. These nanoclusters provide high‑affinity, switch‑like, sequence‑specific DNA interaction, suggesting a novel mechanism for transcriptional regulation by TF nanoclustering.