Integrative genomic and transcriptomic analyses uncover regulatory landscape of symbiotic nitrogen fixation in soybean natural population
Authors: Li, Y., Feng, w., Feng, X., Liu, X., Hao, S., Lian, L., Gao, L., Shao, Y., Chen, H., Chen, Z., Yuan, J., Qin, L., Li, X., Li, X., Wang, X.
Date: 2025-07-23 · Version: 1
DOI: 10.1101/2025.07.18.665310 Category: Plant Biology
Model Organism: Glycine max
▶ AI Summary
The study integrates genome, transcriptome, and chromatin accessibility data from 380 soybean accessions to dissect the genetic and regulatory basis of symbiotic nitrogen fixation (SNF). Using GWAS, TWAS, eQTL mapping, and ATAC-seq, the authors identify key loci, co‑expression modules, and regulatory elements, and validate the circadian clock gene GmLHY1b as a negative regulator of nodulation via CRISPR and CUT&Tag. These resources illuminate SNF networks and provide a foundation for soybean improvement.
symbiotic nitrogen fixation GWAS TWAS eQTL mapping ATAC-seq