Quantitative trait locus mapping of root exudate metabolome in a Solanum lycopersicum Moneymaker x S. pimpinellifolium RIL population and their putative links to rhizosphere microbiome
Authors: Kim, B., Kramer, G., Leite, M. F. A., Snoek, B. L., Zancarini, A., Bouwmeester, H.
The study used untargeted metabolomics and QTL mapping in a tomato recombinant inbred line population to characterize root exudate composition and identify genetic loci controlling specific metabolites. It reveals domestication-driven changes in exudate profiles and links metabolic QTLs with previously reported microbial QTLs, suggesting a genetic basis for shaping the root microbiome.
root exudates untargeted metabolomics quantitative trait loci tomato plant‑microbe interactions
GWAs reveals SUBER GENE1-mediated suberization via Type One Phosphatases
Authors: Han, J.-P., Lefebvre-Legendre, L., Yu, J., Capitao, M. B., Beaulieu, C., Gully, K., Shukla, V., Wu, Y., Boland, A., Nawrath, C., Barberon, M.
Using a forward genetic screen of 284 Arabidopsis thaliana accessions, the study identified extensive natural variation in root endodermal suberin and pinpointed the previously unknown gene SUBER GENE1 (SBG1) as a key regulator. GWAS and protein interaction analyses revealed that SBG1 controls suberin deposition by binding type‑one protein phosphatases (TOPPs), with disruption of this interaction or TOPP loss‑of‑function altering suberin levels, linking the pathway to ABA signaling.
Root growth promotion by Penicillium melinii: mechanistic insights and agricultural applications
Authors: Gutierrez-Manso, L., Devesa-Aranguren, I., Conesa, C. M., Monteoliva-Garcia, G., Gonzalez-Sayer, S., Lozano-Enguita, A., Blasio, F., Ugena, L., Nolasco, J., Vazquez-Mora, A., Levy, C. C. B., Ariel Otero, E., Fernandez-Calvo, P., Moreno-Risueno, M. A., petrik, I., Pencik, A., Reguera, M., Gonzalez-Bodi, S., Huerta-Cepas, J., Sacristan, S., del Pozo, J. C., Cabrera, J.
The study characterizes the endophytic fungus Penicillium melinii, isolated from Arabidopsis thaliana roots, as a plant‑growth‑promoting agent that enhances root architecture and biomass across Arabidopsis, quinoa, and tomato. Integrated phenotypic, transcriptomic, and hormonal analyses reveal that the fungus stimulates auxin‑related pathways and modest stress responses, leading to increased tomato yield in field trials, underscoring its value as a model for root development and a sustainable biostimulant.
The study demonstrates that salinity stress induces a photomorphogenic‑like response in dark‑grown Arabidopsis thaliana seedlings, resulting in reduced apical hook curvature and impaired soil emergence. This phenotype is linked to disrupted asymmetric epidermal cell elongation, decreased auxin signaling and PIN3 abundance on the hook’s concave side, repression of BBX28 expression, and loss of a spatial COP1 gradient, highlighting spatial regulation as a key factor in stress‑affected seedling development.
Identification of a putative RBOHD-FERONIA-CRK10-PIP2;6 plasma membrane complex that interacts with phyB to regulate ROS production in Arabidopsis thaliana
Authors: Mohanty, D., Fichman, Y., Pelaez-Vico, M. A., Myers, R. J., Sealander, M., Sinha, R., Morrow, J., Eckstein, R., Olson, K., Xu, C., An, H., Yoo, C. Y., Zhu, J.-K., Zhao, C., Zandalinas, S. I., Liscum, E., Mittler, R.
The study demonstrates that FERONIA and phytochrome B physically interact with the NADPH oxidase RBOHD, and that FERONIA-mediated phosphorylation of phyB is essential for RBOHD-driven ROS production under excess light stress in Arabidopsis thaliana. Additional membrane proteins CRK10 and PIP2;6 also associate with this complex, forming a plasma‑membrane assembly that integrates multiple signaling pathways to regulate stress‑induced ROS.
reactive oxygen species FERONIA phytochrome B RBOHD excess light stress
SPOROCYTELESS/NOZZLE acts together with MADS-domain transcription factors to regulate an auxin-dependent network controlling the Megaspore Mother Cell development
Authors: Cavalleri, A., Astori, C., Manrique, S., Bruzzaniti, G., Smaczniak, C., Mizzotti, C., Ruiu, A., Spano, M., Movilli, A., Gregis, V., Xu, X., Kaufmann, K., Colombo, L.
The study elucidates the SPL/NZZ‑dependent regulatory pathway governing megaspore mother cell (MMC) differentiation, revealing that SPL/NZZ directly targets genes and interacts with ovule‑identity MADS‑domain transcription factor complexes. Integration of multi‑omics data with genetic complementation and mutant analyses uncovers an auxin‑dependent downstream network that drives MMC formation.
Proline transporters balance the salicylic acid-mediated trade-off between regeneration and immunity in plants
Authors: Yang, L., Xu, D., Belew, Z. M., Cassia Ferreira Dias, N., Wang, L., Zhang, A., Chen, Y.-F. S., Newton, C. J., Kong, F., Zheng, Y., Yao, Y., Brewer, M. T., Teixeira, P. J. P. L., Nour-Eldin, H. H., Xu, D.
The study identifies wound‑induced proline transporters ProT2 and ProT3 as central regulators that link salicylic acid signaling to the suppression of de novo root regeneration (DNRR) via modulation of reactive oxygen species dynamics. Genetic loss of these transporters or pharmacological inhibition of proline transport alleviates SA‑mediated regeneration inhibition across several plant species without compromising disease resistance.
salicylic acid proline transporters de novo root regeneration reactive oxygen species immunity‑regeneration trade‑off
Thermotolerant pollen tube growth is controlled by RALF signaling.
Authors: Althiab Almasaud, R., Ouonkap Yimga, S. V., Ingram, J., Oseguera, Y., Alkassem Alosman, M., Travis, C., Henry, A., Medina, M., Oulhen, N., Wessel, G. M., Delong, A., Pease, J., DaSilva, N., Johnson, M.
The study investigates the molecular basis of heat‑tolerant pollen tube growth in tomato (Solanum lycopersicum) by comparing thermotolerant and sensitive cultivars. Using live imaging, transcriptomics, proteomics, and genetics, the authors identified the Rapid Alkalinization Factor (RALF) signaling pathway as a key regulator of pollen tube integrity under high temperature, with loss of a specific RALF peptide enhancing tube integrity in a thermotolerant cultivar.
The study demonstrates that very long chain sphingolipids in the outer membrane leaflet interdigitate with inner‑leaflet phosphatidylserine, forming a vertical bridge that organizes PS nanodomains and enables auxin‑induced activation of the Rho‑GTPase ROP6. Disruption of sphingolipid biosynthesis disperses these nanodomains, impairing ROP6 signaling, cytoskeletal dynamics, and directional growth, highlighting interleaflet coupling as a key mechanism linking membrane asymmetry to plant signal transduction.
The study combined high-throughput image-based phenotyping with genome-wide association studies to uncover the genetic architecture of tolerance to the spittlebug Aeneolamia varia in 339 interspecific Urochloa hybrids. Six robust QTL were identified for plant damage traits, explaining up to 21.5% of variance, and candidate genes linked to hormone signaling, oxidative stress, and cell‑wall modification were highlighted, providing markers for breeding.