Calcium-dependent protein kinases participate in RBOH-mediated sustained ROS burst during plant immune cell death
Authors: Hino, Y., Yoshioka, M., Adachi, H., Yoshioka, H.
Date: 2025-09-01 · Version: 1
DOI: 10.1101/2025.09.01.672762 Category: Plant Biology
Model Organism: Nicotiana benthamiana
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The study demonstrates that calcium-dependent protein kinases NbCDPK4 and NbCDPK5 directly phosphorylate the NADPH oxidase NbRBOHB at Ser‑123, enhancing sustained ROS production during effector-triggered immunity in Nicotiana benthamiana. Constitutively active CDPKs also upregulate NbRBOHB transcription, and phosphorylation of Ser‑123 is amplified by Ca2+ influx triggered by an autoactive helper NLR (NRC4). These results define a NbCDPK‑NbRBOHB signaling module that links NLR activation to prolonged ROS bursts in ETI.
effector-triggered immunity calcium-dependent protein kinases NADPH oxidase reactive oxygen species Nicotiana benthamiana