The study examined how dual‑purpose hemp (Cannabis sativa) adjusts to different phosphate levels, showing that flower biomass is maintained unless phosphate is completely removed. Integrated physiological measurements and transcriptomic profiling revealed that phosphate is reallocated to flowers via glycolytic bypasses and organic phosphate release, while key regulatory genes followed expected patterns but did not suppress uptake at high phosphate, leading to nitrate depletion that limits growth.

The study characterizes the plant spliceosomal protein AtSF1 in Arabidopsis thaliana, using iCLIP and RNA‑seq to map its in vivo branch point binding sites and demonstrate that loss of AtSF1 causes widespread 3' splice‑site mis‑selection. Structural comparison reveals a plant‑specific domain architecture, and the identified AtSF1 targets are enriched for circadian and defense genes, linking splicing regulation to timing and immunity.

The study identifies MtFTb1 and MtFTb2 as essential, redundant regulators of long‑day flowering in the legume Medicago truncatula, demonstrating that they are required for up‑regulating MtFTa1 under vernalised long‑day conditions. Using CRISPR/Cas9‑generated single and double mutants, the authors show that double mutants are specifically delayed in flowering under long days while retaining vernalization responsiveness, and transcriptomic analyses reveal that MtFTb1/2 activate MADS‑box genes and other flowering regulators.

Using ten Phaeodactylum tricornutum mutant strains with graded constitutive Lhcx1 expression, the study links NPQ induction under high light to physiological outcomes (oxidized QA, increased cyclic electron flow) and extensive transcriptomic reprogramming, affecting nearly half the genome. The approach demonstrates that higher NPQ mitigates PSII damage, boosts ATP production for repair, and drives distinct gene regulatory networks, providing a model framework for dissecting photosynthetic and gene expression integration.

The study identified 12 SABATH methyltransferase genes in the liverwort Marchantia polymorpha and demonstrated that MpSABATH2 is crucial for normal thallus growth and gemma cup formation. Loss‑of‑function mutants displayed developmental phenotypes reminiscent of far‑red light responses, which were linked to gibberellin metabolism and could be partially rescued by inhibiting GA biosynthesis or supplying the GA precursor ent‑kaurenoic acid. These findings suggest that SABATH enzymes independently evolved regulatory roles in land‑plant development.

The study establishes a tractable system using the large bloom-forming diatom Coscinodiscus granii and its natural oomycete parasite Lagenisma coscinodisci, enabling manual isolation of single host cells and stable co-cultures. High‑quality transcriptomes for both partners were assembled, revealing diverse oomycete effectors and a host transcriptional response involving proteases and exosome pathways, while also profiling the co‑occurring heterotrophic flagellate Pteridomonas sp. This tripartite platform provides a unique marine model for dissecting molecular mechanisms of oomycete‑diatom interactions.

The study demonstrates that carbon availability promotes gemma cup formation in Marchantia polymorpha by activating cytokinin signaling, which up‑regulates the transcription factors MpGCAM1 and MpSTG. Pharmacological and genetic manipulations showed that cytokinin accumulation in response to sucrose and high light is sufficient to overcome low‑sucrose repression, and that this pathway operates independently of KAI2A‑MAX2 mediated karrikin signaling. The findings suggest a conserved carbon‑cytokinin interaction governing developmental plasticity across land plants.

The study presents an optimized Agrobacterium-mediated transformation toolkit for Sorghum bicolor that achieves up to 95.7% editing efficiency using CRISPR/Cas9 targeting the SbPDS gene, and demonstrates comparable performance with a PAM‑broadened SpRY variant. This platform enables multiplex genome editing and is positioned for integration of advanced tools such as prime and base editors to accelerate sorghum breeding.

CRISPR/Cas9 knockout of the vacuolar invertase gene (StVInv) in potato enhanced drought resilience, with mutants maintaining higher stomatal conductance, transpiration, and photosynthetic efficiency, leading to improved agronomic water-use efficiency and biomass under water limitation. Metabolomic profiling showed accumulation of galactinol and raffinose, while ABA levels were reduced, indicating altered osmoprotective and hormonal responses that support sustained growth during drought.

The study examined the pre‑activation state of the rice NLR pair Pik‑1 (sensor) and Pik‑2 (helper) when transiently expressed in Nicotiana benthamiana leaves. Both wild‑type and engineered Pik‑1 variants constitutively associate with Pik‑2 to form ~1 MDa hetero‑oligomeric complexes that localize to the plasma membrane in the absence of effector. These results reveal that some NLRs exist as pre‑assembled membrane‑associated complexes prior to pathogen perception.