The study investigates the role of the chromatin regulator MpSWI3, a core subunit of the SWI/SNF complex, in the liverwort Marchantia polymorpha. A promoter mutation disrupts male gametangiophore development and spermiogenesis, causing enhanced vegetative propagation, and transcriptomic analysis reveals that MpSWI3 regulates genes controlling reproductive initiation, sperm function, and asexual reproduction, highlighting its ancient epigenetic role in balancing vegetative and reproductive phases.

The study investigates the role of two ATP-binding cassette transporters, MpABCG1 and MpABCG36, in the sequestration of specialized metabolites within oil bodies of the liverwort Marchantia polymorpha. Loss‑of‑function mutants displayed reduced accumulation of sesquiterpenes and, specifically for MpABCG1, decreased levels of bis‑bibenzyls, while oil‑body formation remained largely unaffected, indicating these transporters are essential for metabolite accumulation rather than organelle biogenesis.

The study characterizes the single-copy S-nitrosoglutathione reductase 1 (MpGSNOR1) in the liverwort Marchantia polymorpha, showing that loss-of-function mutants generated via CRISPR/Cas9 exhibit marked morphological defects and compromised SNO homeostasis and immune responses. These findings indicate that GSNOR-mediated regulation of S‑nitrosylation is an ancient mechanism linking development and immunity in early land plants.

The study identifies an autophagy pathway that degrades plasma membrane-derived clathrin-coated vesicles (CCVs) during hyperosmotic stress, helping maintain membrane tension as cell volume decreases. Using live imaging and correlative microscopy, the authors show that the TPLATE complex subunits AtEH1/Pan1 and AtEH2/Pan1 act as selective autophagy receptors by directly binding ATG8, thereby removing excess membrane under drought or salt conditions.

The study examines how proteasomal degradation of A‑class and B‑class Auxin Response Factors (ARFs) is regulated in the bryophyte Marchantia polymorpha, identifying a key residue required for MpARF2 degradation that is also conserved in MpARF1. While disruption of MpARF2 degradation impairs development across life‑cycle stages, blocking MpARF1 degradation has minimal phenotypic impact, indicating divergent functional integration despite a shared degradation mechanism.

The study generated a comprehensive transcriptome assembly for the macauba palm (Acrocomia aculeata) across seven distinct organs, annotating 42.85% of transcripts and identifying organ‑specific expression patterns. Comparative analyses revealed macauba‑unique gene families and numerous drought‑responsive genes, while root samples uncovered a notable presence of arbuscular mycorrhizal fungal transcripts, underscoring the importance of symbiotic interactions and stress signaling pathways.

The study investigates the function of two GT47B arabinan arabinosyltransferases in the liverwort Marchantia polymorpha, generating loss‑of‑function and overexpression lines to assess cell wall composition. Using CoMPP, glycosyl linkage analysis, and LM6 immunolabelling, the authors found that MpARADL2 mutants have reduced 1,5‑L‑arabinan epitopes in elaters despite unchanged overall 5‑linked Araf levels, suggesting additional enzymes compensate in thallus tissue. Attempts to express and purify the enzymes in HEK293 cells failed, implying a clade‑specific solubility requirement and highlighting the need to identify interacting partners.

The study characterizes the composition and structure of cell wall glycans in eight tissue types of the liverwort Marchantia polymorpha, revealing both typical land‑plant features and unique traits such as abundant (1,5)-arabinan in sporophytes and low overall pectin levels. Comparative genomic analysis shows a diversified glycosyltransferase repertoire relative to Arabidopsis, and the authors created a Gateway‑compatible library of 93 M. polymorpha GTs to facilitate future functional studies.

The authors identified MpCAFA, a protein combining CAPS-like and FAP115-like domains, as a key factor for rapid ciliary swimming in the liverwort Marchantia polymorpha spermatozoids. Loss-of-function mutants displayed markedly reduced swimming speed despite normal axoneme structure, chemotaxis, and fertility, and these defects were rescued by a MpCAFA‑mCitrine fusion that localized along the entire cilium. Both the CAPS-like and FAP115-like regions are required for MpCAFA’s function and ciliary targeting, establishing it as a major ciliary protein and a marker for visualizing spermatozoid motility.

The study investigated meristem activation in the liverwort Marchantia polymorpha, revealing that simulated shade causes alternating inactivity of meristems. Transcriptomic comparison of active versus inactive meristems identified the cytochrome P450 monooxygenase MpCYP78E1 as an inhibitor of meristem activity and initiation, with loss- and gain-of-function mutants confirming its regulatory role in shoot branching architecture.