The study investigates the function of two GT47B arabinan arabinosyltransferases in the liverwort Marchantia polymorpha, generating loss‑of‑function and overexpression lines to assess cell wall composition. Using CoMPP, glycosyl linkage analysis, and LM6 immunolabelling, the authors found that MpARADL2 mutants have reduced 1,5‑L‑arabinan epitopes in elaters despite unchanged overall 5‑linked Araf levels, suggesting additional enzymes compensate in thallus tissue. Attempts to express and purify the enzymes in HEK293 cells failed, implying a clade‑specific solubility requirement and highlighting the need to identify interacting partners.

The study characterizes the composition and structure of cell wall glycans in eight tissue types of the liverwort Marchantia polymorpha, revealing both typical land‑plant features and unique traits such as abundant (1,5)-arabinan in sporophytes and low overall pectin levels. Comparative genomic analysis shows a diversified glycosyltransferase repertoire relative to Arabidopsis, and the authors created a Gateway‑compatible library of 93 M. polymorpha GTs to facilitate future functional studies.

The study performed daily large-scale glycome, transcriptome, and proteome profiling of developing fibers from the two cultivated cotton species, Gossypium barbadense and G. hirsutum, across primary and secondary cell wall stages. It identified delayed cellulose accumulation and distinct compositions of xyloglucans, homogalacturonans, rhamnogalacturonan‑I, and heteroxylans in G. barbadense, along with higher expression of specific glycosyltransferases and expansins, suggesting these molecular differences underlie the superior fiber length and strength of G. barbadense.

The authors identified MpCAFA, a protein combining CAPS-like and FAP115-like domains, as a key factor for rapid ciliary swimming in the liverwort Marchantia polymorpha spermatozoids. Loss-of-function mutants displayed markedly reduced swimming speed despite normal axoneme structure, chemotaxis, and fertility, and these defects were rescued by a MpCAFA‑mCitrine fusion that localized along the entire cilium. Both the CAPS-like and FAP115-like regions are required for MpCAFA’s function and ciliary targeting, establishing it as a major ciliary protein and a marker for visualizing spermatozoid motility.

The study investigated meristem activation in the liverwort Marchantia polymorpha, revealing that simulated shade causes alternating inactivity of meristems. Transcriptomic comparison of active versus inactive meristems identified the cytochrome P450 monooxygenase MpCYP78E1 as an inhibitor of meristem activity and initiation, with loss- and gain-of-function mutants confirming its regulatory role in shoot branching architecture.

The study examines how the SnRK1 catalytic subunit KIN10 integrates carbon availability with root growth regulation in Arabidopsis thaliana. Loss of KIN10 reduces glucose‑induced inhibition of root elongation and triggers widespread transcriptional reprogramming of metabolic and hormonal pathways, notably affecting auxin and jasmonate signaling under sucrose supplementation. These findings highlight KIN10 as a central hub linking energy status to developmental and environmental cues in roots.

The study demonstrates that MYB‑bHLH‑WDR transcriptional complexes (MBW) are present in the liverwort Marchantia polymorpha, indicating that such complexes originated before the diversification of land plants. Functional analyses reveal that two MYB paralogs, MpMYB14 and MpMYB02, rely on a single bHLH partner (MpbHLH12) to regulate flavonoid biosynthesis and liverwort‑specific oil body maturation, suggesting an ancestral role in pigment production and a derived role in organelle development.

The study evaluated how acute heat stress affects early-stage rice seedlings, identifying a critical temperature threshold that impairs growth. Transcriptomic profiling of shoots and roots revealed ethylene‑responsive factors (ERFs) as central regulators, with ethylene and jasmonic acid acting upstream, and pre‑treatment with these hormones mitigated heat damage. These findings highlight ERF‑hormone interaction networks as targets for improving rice heat resilience.

The authors adapted OpenPlant kit CRISPR/Cas9 tools to enable multiplex gRNA expression from a single transcript using tRNA sequences in the liverwort Marchantia polymorpha, markedly enhancing editing efficiency and scalability. They coupled this vector system with a simplified, optimized thallus transformation protocol, providing a rapid and versatile platform for generating CRISPR/Cas9 mutants and advancing functional genomics in this model species.

Using the Euphorbia peplus genome, the authors performed organ‑specific transcriptomic profiling of the cyathium and combined it with gene phylogenies and dN/dS analysis to investigate floral‑development gene families. They found distinct SEP1 paralog expression, lack of E‑class gene duplications typical of other pseudanthia, and divergent expression patterns for CRC, UFO, LFY, AP3, and PI, suggesting unique developmental pathways in Euphorbia.