A tRNA-gRNA multiplexing system for CRISPR genome editing in Marchantia polymorpha.
Authors: Frangedakis, E., Yelina, N., Eeda, S. K., Romani, F., Fragkidis, A., Haseloff, J., Hibberd, J.
Date: 2025-04-20 · Version: 1
DOI: 10.1101/2025.04.18.649274 Category: Plant Biology
Model Organism: Marchantia polymorpha
▶ AI Summary
The authors adapted OpenPlant kit CRISPR/Cas9 tools to enable multiplex gRNA expression from a single transcript using tRNA sequences in the liverwort Marchantia polymorpha, markedly enhancing editing efficiency and scalability. They coupled this vector system with a simplified, optimized thallus transformation protocol, providing a rapid and versatile platform for generating CRISPR/Cas9 mutants and advancing functional genomics in this model species.
CRISPR/Cas9 multiplex gRNA expression tRNA-mediated processing Marchantia polymorpha thallus transformation