Genetius

The study examined how osmotic stress and cytosolic Ca²⁺ signaling regulate autophagy in plants by monitoring the dynamics of RFP‑tagged ATG8i. Both stimuli altered the accumulation of RFP‑ATG8i‑labeled autophagosomes in an organ‑specific way, and colocalization with the ER marker HDEL indicated that ATG8i participates in ER‑phagy during stress.

Reduced activity of methylthioadenosine (MTA) nucleosidase causes MTA over‑accumulation in reproductive tissues, leading to lowered cysteine, methionine, and S‑adenosylmethionine levels and altered sulfur and energy metabolism. These metabolic disturbances trigger misregulation of cell‑cycle progression, widespread down‑regulation of developmental genes, and genome‑wide changes in DNA methylation patterns, highlighting the extensive role of MTA recycling in plant growth and methyl‑index maintenance.

The study reveals that the bacterial pathogen Pseudomonas syringae suppresses host plant translation by targeting processing bodies (P‑bodies) through two liquid-like effectors, linking this repression to the ER stress response. It further demonstrates that autophagic clearance of P‑bodies is essential for balancing translationally active and inactive mRNAs, uncovering new connections among translation, ER stress, and autophagy during plant immunity.