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Integrative analysis of plant responses to a combination of water deficit, heat stress and eCO2 reveals a role for OST1 and SLAH3 in regulating stomatal responses

Authors: Pelaez-Vico, M. A., Sinha, R., Ghani, A., Lopez-Climent, M. F., Joshi, T., Fritschi, F. B., Zandalinas, S. I., Mittler, R.

Date: 2025-05-11 · Version: 1
DOI: 10.1101/2025.05.07.652739

Category: Plant Biology

Model Organism: Arabidopsis thaliana

AI Summary

The study examined how Arabidopsis thaliana integrates physiological, genetic, hormonal, and transcriptomic responses to combined water deficit, heat stress, and elevated CO2. Results show that stomatal aperture under these complex stress combinations is governed by a specific set of regulators, including nitric oxide, OPEN STOMATA 1, and the SLAH3 anion channel, distinct from those active under simpler stress conditions. This reveals a hierarchical stomatal stress code that could inform future research on plant resilience to global change.

Global Change Factor combination stomatal aperture regulation Arabidopsis thaliana water deficit heat stress

Arabidopsis root lipid droplets are hubs for membrane homeostasis under heat stress, and triterpenoid synthesis and storage.

Authors: Scholz, P., Dabisch, J., Clews, A. C., Niemeyer, P. W., Vilchez, A. C., Lim, M. S. S., Sun, S., Hembach, L., Dreier, F., Blersch, K., Preuss, L., Bonin, M., Lesch, E., Iwai, Y., Shimada, T., Eirich, J., Finkemeier, I., Gutbrod, K., Doermann, P., Wang, Y., Mullen, R. T., Ischebeck, T.

Date: 2025-03-26 · Version: 1
DOI: 10.1101/2025.03.24.644787

Category: Plant Biology

Model Organism: Arabidopsis thaliana

AI Summary

The study examined how heat stress alters lipid droplet (LD) number and composition in Arabidopsis thaliana roots, revealing degradation of membrane lipids and accumulation of TAGs and LDs. Proteomic and lipidomic analyses of LDs from a specific Arabidopsis mutant identified novel LD-associated proteins, including triterpene biosynthetic enzymes, whose substrates and products also accumulate in LDs, indicating LDs function as both sinks and sources during stress‑induced membrane remodeling and specialized metabolism.

lipid droplets heat stress Arabidopsis thaliana roots triterpene biosynthesis lipidomics
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