Clathrin-coated vesicles are targeted for selective autophagy during osmotic stress.
Authors: dragwidge, j., Buridan, M., Kraus, J., Kosuth, T., Chambaud, C., Brocard, L., Yperman, K., Mylle, E., Vandorpe, M., Eeckhout, D., De Jaeger, G., Pleskot, R., Bernard, A., Van Damme, D.
The study identifies an autophagy pathway that degrades plasma membrane-derived clathrin-coated vesicles (CCVs) during hyperosmotic stress, helping maintain membrane tension as cell volume decreases. Using live imaging and correlative microscopy, the authors show that the TPLATE complex subunits AtEH1/Pan1 and AtEH2/Pan1 act as selective autophagy receptors by directly binding ATG8, thereby removing excess membrane under drought or salt conditions.