Sixteen upland rice varieties were evaluated under three irrigation regimes (100%, 70%, and 50% field capacity) with additional six‑day water withholding to simulate moderate and severe drought. Yield losses ranged from 35% to 78% depending on stress level, and varieties Dawk Kha, Khao/Sai, and Dawk Pa‑yawm showed the greatest stability, suggesting they are promising for breeding drought‑resilient upland rice.
upland rice drought stress field capacity irrigation yield loss varietal stability
The influence of heavy metal stress on the evolutionary transition of teosinte to maize
Authors: Acosta Bayona, J. J., Vallebueno-Estrada, M., Vielle-Calzada, J.-P.
The study tests whether heavy‑metal stress contributed to maize domestication by exposing teosinte (Zea mays ssp. parviglumis) and the Palomero toluqueno landrace to sublethal copper and cadmium, then analysing genetic diversity, selection signatures, and transcriptomic responses of three chromosome‑5 heavy‑metal response genes (ZmHMA1, ZmHMA7, ZmSKUs5). Results reveal strong positive selection on these genes, heavy‑metal‑induced phenotypes resembling modern maize, and up‑regulation of Tb1, supporting a role for volcanic‑derived metal stress in early maize evolution.
heavy metal stress maize domestication Zea mays positive selection Tb1
In vivo binding by Arabidopsis SPLICING FACTOR 1 shifts 3' splice site choice, regulating circadian rhythms and immunity in plants
Authors: Agrofoglio, Y. C., Iglesias, M. J., de Leone, M. J., Hernando, C. E., Lewinski, M., Torres, S. B., Contino, G., Yanovsky, M. J., Staiger, D., Mateos, J. L.
The study characterizes the plant spliceosomal protein AtSF1 in Arabidopsis thaliana, using iCLIP and RNA‑seq to map its in vivo branch point binding sites and demonstrate that loss of AtSF1 causes widespread 3' splice‑site mis‑selection. Structural comparison reveals a plant‑specific domain architecture, and the identified AtSF1 targets are enriched for circadian and defense genes, linking splicing regulation to timing and immunity.
alternative splicing branch point recognition AtSF1 circadian clock regulation plant immunity
FLOWERING LOCUS T genes MtFTb1 and MtFTb2 act redundantly to promote flowering under long days in Medicago truncatula
The study identifies MtFTb1 and MtFTb2 as essential, redundant regulators of long‑day flowering in the legume Medicago truncatula, demonstrating that they are required for up‑regulating MtFTa1 under vernalised long‑day conditions. Using CRISPR/Cas9‑generated single and double mutants, the authors show that double mutants are specifically delayed in flowering under long days while retaining vernalization responsiveness, and transcriptomic analyses reveal that MtFTb1/2 activate MADS‑box genes and other flowering regulators.
flowering time FT genes Medicago truncatula CRISPR/Cas9 long‑day photoperiod
Network Inference Reveals Distinct Transcriptional Regulation in Barley against Drought and Fusarium Head Blight
Authors: Steidele, C. E., Kersting, J., Hoheneder, F., List, M., Hueckelhoven, R.
The study used Weighted Gene Correlation Network Analysis (WGCNA) and GENIE3 to construct co‑expression modules and gene regulatory networks (GRNs) in barley subjected to Fusarium head blight and drought stress. Integration of these approaches highlighted overlapping regulatory patterns, pinpointing WRKY transcription factors as central to FHB response, while bHLH and NAC family members showed stress‑specific roles. Promoter motif enrichment further validated predicted TF‑target interactions, offering candidate regulators for future functional validation.
The authors used computational simulations of plant cellular metabolism under historical atmospheric conditions to demonstrate that reduced CO₂ and increased aridity can drive the evolutionary transition from C₃ to CAM photosynthesis. Their results suggest that while future elevated CO₂ may favor a reversion to C₃-like behavior, drought consistently promotes CAM regardless of CO₂ or temperature, and a minimum O₂ level is required for nocturnal respiration in CAM.
The study presents an optimized Agrobacterium-mediated transformation toolkit for Sorghum bicolor that achieves up to 95.7% editing efficiency using CRISPR/Cas9 targeting the SbPDS gene, and demonstrates comparable performance with a PAM‑broadened SpRY variant. This platform enables multiplex genome editing and is positioned for integration of advanced tools such as prime and base editors to accelerate sorghum breeding.
The study used paired whole‑genome bisulphite sequencing and RNA‑seq on wheat landraces to investigate how DNA methylation patterns change during drought stress, revealing antagonistic trends across cytosine contexts and a key demethylation role for ROS1a family members. Gene‑body methylation correlated positively with expression but negatively with stress‑responsive changes, while drought‑induced hyper‑methylation of specific transposable elements, especially the RLX_famc9 LTR retrotransposon, appears to modulate downstream gene regulation via siRNA precursors.
drought stress DNA methylation Triticum aestivum ROS1a demethylase transposable elements
Vacuolar invertase knockout enhances drought tolerance in potato plants
Authors: Roitman, M., Teper-Bamnolker, P., Doron-Faigenboim, A., Sikron, N., Fait, A., Vrobel, O., Tarkowski, P., Moshelion, M., Bocobza, S., Eshel, D.
CRISPR/Cas9 knockout of the vacuolar invertase gene (StVInv) in potato enhanced drought resilience, with mutants maintaining higher stomatal conductance, transpiration, and photosynthetic efficiency, leading to improved agronomic water-use efficiency and biomass under water limitation. Metabolomic profiling showed accumulation of galactinol and raffinose, while ABA levels were reduced, indicating altered osmoprotective and hormonal responses that support sustained growth during drought.
drought stress vacuo lar invertase knockout CRISPR/Cas9 raffinose family oligosaccharides water-use efficiency
Authors: Baer, M., Zhong, Y., Yu, B., Tian, T., He, X., Gu, L., Huang, X., Gallina, E., Metzen, I. E., Bucher, M., Song, R., Gutjahr, C., SU, Z., Moya, Y., von Wiren, N., Zhang, L., Yuan, L., Shi, Y., Wang, S., Qi, W., Baer, M., Zhao, Z., Li, C., Li, X., Hochholdinger, F., Yu, P.
The study uncovers how arbuscular mycorrhizal (AM) fungi induce lateral root formation in maize by activating ethylene‑responsive transcription factors (ERFs) that regulate pericycle cell division and reshape flavonoid metabolism, lowering inhibitory flavonols. It also shows that the rhizobacterium Massilia collaborates with AM fungi, degrading flavonoids and supplying auxin, thereby creating an integrated ethylene‑flavonoid‑microbe signaling network that can be harnessed to improve nutrient uptake and crop sustainability.