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Arabidopsis root lipid droplets are hubs for membrane homeostasis under heat stress, and triterpenoid synthesis and storage.

Authors: Scholz, P., Dabisch, J., Clews, A. C., Niemeyer, P. W., Vilchez, A. C., Lim, M. S. S., Sun, S., Hembach, L., Dreier, F., Blersch, K., Preuss, L., Bonin, M., Lesch, E., Iwai, Y., Shimada, T., Eirich, J., Finkemeier, I., Gutbrod, K., Doermann, P., Wang, Y., Mullen, R. T., Ischebeck, T.

Date: 2025-03-26 · Version: 1
DOI: 10.1101/2025.03.24.644787

Category: Plant Biology

Model Organism: Arabidopsis thaliana

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The study examined how heat stress alters lipid droplet (LD) number and composition in Arabidopsis thaliana roots, revealing degradation of membrane lipids and accumulation of TAGs and LDs. Proteomic and lipidomic analyses of LDs from a specific Arabidopsis mutant identified novel LD-associated proteins, including triterpene biosynthetic enzymes, whose substrates and products also accumulate in LDs, indicating LDs function as both sinks and sources during stress‑induced membrane remodeling and specialized metabolism.

lipid droplets heat stress Arabidopsis thaliana roots triterpene biosynthesis lipidomics
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