MdBRC1 and MdFT2 Interaction Fine-Tunes Bud Break Regulation in Apple
Authors: Gioppato, H. A., Estevan, J., Al Bolbol, M., Soriano, A., Garighan, J., Jeong, K., Georget, C., Soto, D. G., El Khoury, S., Falavigna, V. d. S., George, S., Perales, M., Andres, F.
The study identifies the transcription factor MdBRC1 as a key inhibitor of bud growth during the ecodormancy phase in apple (Malus domestica), directly regulating dormancy‑associated genes and interacting with the flowering promoter MdFT2 to modulate bud break. Comparative transcriptomic analysis and gain‑of‑function experiments in poplar demonstrate that MdFT2 physically binds MdBRC1, attenuating its repressive activity and acting as a molecular switch for the transition to active growth.
The study introduced full-length SOC1 genes from maize and soybean, and a partial SOC1 gene from blueberry, into tomato plants under constitutive promoters. While VcSOC1K and ZmSOC1 accelerated flowering, all three transgenes increased fruit number per plant mainly by promoting branching, and transcriptomic profiling revealed alterations in flowering, growth, and stress‑response pathways.
The study used transcriptomic and lipidomic profiling to investigate how chia (Salvia hispanica) leaves respond to short‑term (3 h) and prolonged (27 h) heat stress at 38 °C, revealing rapid activation of calcium‑signaling and heat‑shock pathways and reversible changes in triacylglycerol levels. Nearly all heat‑responsive genes returned to baseline expression after 24 h recovery, highlighting robust thermotolerance mechanisms that could inform improvement of other oilseed crops.
Arabidopsis lines with modified ascorbate concentrations reveal a link between ascorbate and auxin biosynthesis
Authors: Fenech, M., Zulian, V., Moya-Cuevas, J., Arnaud, D., Morilla, I., Smirnoff, N., Botella, M. A., Stepanova, A. N., Alonso, J. M., Martin-Pizarro, C., Amorim-Silva, V.
The study used Arabidopsis thaliana mutants with low (vtc2, vtc4) and high (vtc2/OE-VTC2) ascorbate levels to examine how ascorbate concentration affects gene expression and cellular homeostasis. Transcriptomic analysis revealed that altered ascorbate levels modulate defense and stress pathways, and that TAA1/TAR2‑mediated auxin biosynthesis is required for coping with elevated ascorbate in a light‑dependent manner.
Comparative multi-omics profiling of Gossypium hirsutum and Gossypium barbadense fibers at high temporal resolution reveals key differences in polysaccharide composition and associated glycosyltransferases
Authors: Swaminathan, S., Lee, Y., Grover, C. E., DeTemple, M. F., Mugisha, A. S., Sichterman, L. E., Yang, P., Xie, J., Wendel, J. F., Szymanski, D. B., Zabotina, O. A.
The study performed daily large-scale glycome, transcriptome, and proteome profiling of developing fibers from the two cultivated cotton species, Gossypium barbadense and G. hirsutum, across primary and secondary cell wall stages. It identified delayed cellulose accumulation and distinct compositions of xyloglucans, homogalacturonans, rhamnogalacturonan‑I, and heteroxylans in G. barbadense, along with higher expression of specific glycosyltransferases and expansins, suggesting these molecular differences underlie the superior fiber length and strength of G. barbadense.
The study evaluated how acute heat stress affects early-stage rice seedlings, identifying a critical temperature threshold that impairs growth. Transcriptomic profiling of shoots and roots revealed ethylene‑responsive factors (ERFs) as central regulators, with ethylene and jasmonic acid acting upstream, and pre‑treatment with these hormones mitigated heat damage. These findings highlight ERF‑hormone interaction networks as targets for improving rice heat resilience.
The autophagy-related genes AtATG5 and AtATG7 influence reserve mobilisation and responses to ABA during seed germination in Arabidopsis thaliana
Authors: Contreras, E., Sanchez-Vicente, I., Pastor-Mora, E., Aylon-Rodriguez, M., Gonzalez-Ceballos, M., Delgado-Gutierrez, M. A., Lorenzo, O., Vicente-Carbajosa, J., Iglesias-Fernandez, R.
The study examines how autophagy-related genes AtATG5 and AtATG7 influence Arabidopsis seed germination and ABA responses, revealing that atg5 and atg7 mutants germinate more slowly and display altered lipid droplet and protein storage vacuole organization. Transcriptomic and immunolocalization analyses show delayed ABI5 decay and a direct interaction between ATG8 and the autophagy machinery, implicating autophagy in seed reserve mobilization via transcription factor turnover.
Using the Euphorbia peplus genome, the authors performed organ‑specific transcriptomic profiling of the cyathium and combined it with gene phylogenies and dN/dS analysis to investigate floral‑development gene families. They found distinct SEP1 paralog expression, lack of E‑class gene duplications typical of other pseudanthia, and divergent expression patterns for CRC, UFO, LFY, AP3, and PI, suggesting unique developmental pathways in Euphorbia.
The study shows that heatwaves impair the ability of apple (Malus domestica) to mount ASM‑induced immunity against fire blight and apple scab, leading to a loss of protective gene expression. Transcriptomic analysis revealed a broad suppression of ASM‑regulated defense and other biological processes under high temperature, identifying thermo‑sensitive resistance and susceptibility marker genes. The findings highlight that elevated temperature both weakens plant defenses and creates a more favorable environment for pathogens.
The study identified a major QTL (qDTH3) on chromosome 3 responsible for a 7‑10‑day earlier heading phenotype in the rice line SM93, using QTL‑seq, KASP genotyping, association mapping, and transcriptomic analysis to fine‑map the locus to a 2.53 Mb region and pinpoint candidate genes. SNP markers linked to these genes were proposed as tools for breeding early‑maturing, climate‑resilient rice varieties.