The study generated two allotriploid Brassica hybrids (ArAnCn) to investigate asymmetric subgenome dominance, finding that the Cn subgenome dominates despite the An subgenome showing highest expression levels. Increased density of accessible chromatin regions (ACRs) in the Cn subgenome correlates with dominant gene expression, while changes in CHH methylation and specific RNA‑directed DNA methylation pathway mutants affect subgenome bias.
Assembly and annotation of Solanum dulcamara and Solanum nigrum plant genomes, two nightshades with different susceptibilities to Ralstonia solanacearum
Authors: Franco Ortega, S., James, S. R., Gilbert, L., Hogg, K., Stevens, H., Daff, J., Friman, V. P., Harper, A. L.
The study generated de‑novo genome assemblies for the resistant wild relative Solanum dulcamara and the susceptible Solanum nigrum using a hybrid Oxford Nanopore and Illumina sequencing strategy. Comparative genomic analyses identified auxin‑transport genes and novel pattern recognition receptor orthogroups unique to resistant species, as well as differential gene‑body methylation that may underlie resistance to Ralstonia solanacearum.