Genetius

The study visualizes subcellular dynamics following activation of the NRC4 resistosome, showing that NRC4 enrichment at the plasma membrane triggers calcium influx, followed by sequential disruption of mitochondria, plastids, endoplasmic reticulum, and cytoskeleton, culminating in plasma membrane rupture and cell death. These observations define a temporally ordered cascade of organelle and membrane events that execute plant immune cell death.

The study finely dissected shoot stem cell–enriched tissues from maize and Arabidopsis thaliana and optimized single‑cell RNA‑seq protocols to reliably capture CLAVATA3 and WUSCHEL‑expressing cells. Cross‑species comparison and functional validation, including spatial transcriptomics and mutant analyses, revealed conserved ribosome‑associated RNA‑binding proteins and sugar‑kinase families as key regulators linked to shoot development and yield traits.

The study examined how tricarboxylic acid (TCA) cycle metabolites influence drought tolerance in grapevine and Arabidopsis, finding that malate uniquely triggers stomatal closure via elevations in cytosolic Ca2+ and activation of the SLAC1 anion channel. G-proteins were shown to be essential for malate‑mediated signaling, linking metabolic changes to drought response through a second‑messenger cascade.