A biparental Vicia faba mapping population was screened under glasshouse conditions for resistance to a mixture of Fusarium avenaceum and Fusarium oxysporum, revealing several families with moderate to high resistance. Using the Vfaba_v2 Axiom SNP array, a high-density linkage map of 6,755 SNPs was constructed, enabling the identification of a major QTL on linkage group 4 associated with partial resistance to foot and root rot.
The study demonstrates that RNA extracted from herbarium specimens can be used to generate high‑quality transcriptomes, comparable to those from fresh or silica‑dried samples. By assembling and comparing transcriptomes across specimen types, the authors validated a plant immune receptor synthesized from a 1956 collection, proving archival RNA’s utility for functional genomics. These findings challenge the prevailing view that herbarium RNA is unsuitable for transcriptomic analyses.
A maize near-isogenic line population designed for gene discovery and characterization of allelic effects
Authors: Zhong, T., Mullens, A., Morales, L., Swarts, K., Stafstrom, W., He, Y., Sermons, S., Yang, Q., Lopez-Zuniga, L. O., Rucker, E., Thomason, W., Nelson, R., Jamann, T. M., Balint-Kurti, P., Holland, J. B.
The study characterized 1,264 maize near‑isogenic lines derived from 18 donor inbreds crossed to the recurrent parent B73, using genotyping‑by‑sequencing and SNP‑chip data to detect 2,972 introgression segments via a novel hidden Markov model pipeline. Disease phenotyping enabled QTL mapping for foliar disease resistance, revealing extensive allelic variation among donor lines, and establishing the nNIL population as a valuable resource for dissecting complex traits in maize.