The study introduced full-length SOC1 genes from maize and soybean, and a partial SOC1 gene from blueberry, into tomato plants under constitutive promoters. While VcSOC1K and ZmSOC1 accelerated flowering, all three transgenes increased fruit number per plant mainly by promoting branching, and transcriptomic profiling revealed alterations in flowering, growth, and stress‑response pathways.
Arabidopsis lines with modified ascorbate concentrations reveal a link between ascorbate and auxin biosynthesis
Authors: Fenech, M., Zulian, V., Moya-Cuevas, J., Arnaud, D., Morilla, I., Smirnoff, N., Botella, M. A., Stepanova, A. N., Alonso, J. M., Martin-Pizarro, C., Amorim-Silva, V.
The study used Arabidopsis thaliana mutants with low (vtc2, vtc4) and high (vtc2/OE-VTC2) ascorbate levels to examine how ascorbate concentration affects gene expression and cellular homeostasis. Transcriptomic analysis revealed that altered ascorbate levels modulate defense and stress pathways, and that TAA1/TAR2‑mediated auxin biosynthesis is required for coping with elevated ascorbate in a light‑dependent manner.
The study demonstrates that the microtubule‑associated protein WDL4 is essential for PhyB‑dependent thermomorphogenic and photomorphogenic responses in Arabidopsis, as wdl4-3 mutants mimic phyB loss‑of‑function phenotypes under varying temperatures and light conditions. Genetic analyses reveal that PIF4 activity is required for wdl4-3 hypocotyl hyper‑elongation, and while exogenous auxin can rescue pif4‑related defects, it does not restore the wdl4-3 specific elongation, indicating additional regulatory layers.