The genetic architecture of leaf vein density traits and its importance for photosynthesis in maize
Authors: Coyac-Rodriguez, J. L., Perez-Limon, S., Hernandez-Jaimes, E., Hernandez-Coronado, M., Camo-Escobar, D., Alonso-Nieves, A. L., Ortega-Estrada, M. d. J., Gomez-Capetillo, N., Sawers, R. J., Ortiz-Ramirez, C. H.
Using diverse Mexican maize varieties and a MAGIC population, the study demonstrated that leaf vein density is both variable and plastic, correlating positively with photosynthetic rates for small intermediate veins and increasing under heat in drought-adapted lines. Twelve QTLs linked to vein patterning were identified, highlighting candidate genes for intermediate vein development and shedding light on the evolution of high-efficiency C4 leaf architecture.
The study introduces a hybrid modeling framework that integrates a logistic ordinary differential equation with a Long Short-Term Memory neural network to form a Physics-Informed Neural Network (PINN) for predicting wheat plant height. Using only time and temperature as inputs, the PINN outperformed other longitudinal growth models, achieving the lowest average RMSE and reduced variability across multiple random initializations. The results suggest that embedding biological growth constraints within data‑driven models can substantially improve prediction accuracy for plant traits.
The study evaluated how stomatal anatomy and physiological efficiency influence wheat heat tolerance across multi‑environment field trials with 200 genotypes, using early versus delayed sowing to impose temperature stress. Findings revealed a decoupling between anatomical capacity (gsmax) and actual conductance (gs, gse) under heat, plastic shifts toward smaller, denser stomata, and identified 125 QTL linked to stomatal traits, suggesting targets for breeding climate‑resilient wheat.
Evolution of HMA-integrated tandem kinases accompanied by expansion of target pathogens
Authors: Asuke, S., Tagle, A. G., Hyon, G.-S., Koizumi, S., Murakami, T., Horie, A., Niwamoto, D., Katayama, E., Shibata, M., Takahashi, Y., Islam, M. T., Matsuoka, Y., Yamaji, N., Shimizu, M., Terauchi, R., Hisano, H., Sato, K., Tosa, Y.
The study cloned the resistance genes Rmo2 and Rwt7 from barley and wheat, revealing them as orthologous tandem kinase proteins (TKPs) with an N‑terminal heavy metal‑associated (HMA) domain. Domain‑swapping experiments indicated that the HMA domain dictates effector specificity, supporting a model of TKP diversification into paralogs and orthologs that recognize distinct pathogen effectors.
The authors used a bottom‑up thermodynamic modelling framework to investigate how plants decode calcium signals, starting from Ca2+ binding to EF‑hand proteins and extending to higher‑order decoding modules. They identified six universal Ca2+-decoding modules that can explain variations in calcium sensitivity among kinases and provide a theoretical basis for interpreting calcium signal amplitude and frequency in plant cells.
Mutations in the plastid division gene PARC6 and the granule initiation gene BGC1 were combined to generate wheat plants with dramatically enlarged A-type starch granules, some exceeding 50 µm, without affecting plant growth, grain size, or overall starch content. The parc6 bgc1 double mutant was evaluated in both glasshouse and field trials, and the giant granules displayed altered viscosity and pasting temperature, offering novel functional properties for food and industrial applications.
Glycosylated diterpenes associate with early containment of Fusarium culmorum infection across wheat (Triticum aestivum L.) genotypes under field conditions
Authors: Pieczonka, S. A., Dick, F., Bentele, M., Ramgraber, L., Prey, L., Kupczyk, E., Seidl-Schulz, J., Hanemann, A., Noack, P. O., Asam, S., Schmitt-Kopplin, P., Rychlik, M.
The researchers performed a large‑scale field trial with 105 wheat (Triticum aestivum) genotypes inoculated by Fusarium culmorum, combining quantitative deoxynivalenol (DON) profiling and untargeted metabolomics to uncover molecular signatures of infection. Sesquiterpene‑derived metabolites tracked toxin accumulation, whereas glycosylated diterpene conjugates were enriched in low‑DON samples, indicating a potential defensive metabolic pathway.
Characterization of a dominant SmNac-like gene as a candidate for photosensitivity in the fruit peel of eggplant
Authors: Gomis-Cebolla, J., Manrique, S., Arrones, A., Toledo-Tolgar, M. D., Luna, J., Baraja-Fonseca, V., Sanchez-Pascual, J., Gimeno-Paez, E., Plazas, M., Gramazio, P., Vilanova, S., Prohens, J.
The study identified that fruit photosensitivity in eggplant is governed by a single dominant gene, with QTLs clustering at the distal end of chromosome 10 (84.1-87.9 Mb). Bulked segregant analysis sequencing and RNA‑seq highlighted the SmNAC1‑like transcription factor as a likely regulator of anthocyanin accumulation, though no coding sequence mutations were detected, suggesting regulatory control at another level.
The study investigates the wheat Pm3 NLR allelic series, revealing that near-identical Pm3d and Pm3e alleles confer broad-spectrum resistance by recognizing multiple, structurally diverse powdery mildew effectors. Using chimeric NLR constructs, the authors pinpoint specificity-determining polymorphisms and demonstrate that engineered combinations of Pm3d and Pm3e further expand effector recognition, showcasing the potential for durable wheat protection through NLR engineering.
Novel substrate affinity of FaCCR1 and FaCCR1/FaOCT4 expression control the content of medium-chain esters in strawberry fruit
Authors: Roldan-Guerra, F. J., Amorim-Silva, V., Jimenez, J., Mari-Albert, A., Torreblanca, R., Ruiz del Rio, J., Botella, M. A., Granell, A., Sanchez-Sevilla, J. F., Castillejo, C., Amaya, I.
The study identified a major QTL on chromosome 6A that accounts for 40% of variation in medium-chain ester (MCE) levels in strawberry fruit, pinpointing FaCCR1 and FaOCT4 as the causal genes. Functional validation through subcellular localization, transient overexpression, enzymatic assays, and molecular docking demonstrated that FaCCR1 also catalyzes MCE precursor reactions, and a KASP marker in FaOCT4 was developed for breeding fragrant cultivars.