The study generated de‑novo genome assemblies for the resistant wild relative Solanum dulcamara and the susceptible Solanum nigrum using a hybrid Oxford Nanopore and Illumina sequencing strategy. Comparative genomic analyses identified auxin‑transport genes and novel pattern recognition receptor orthogroups unique to resistant species, as well as differential gene‑body methylation that may underlie resistance to Ralstonia solanacearum.

The study demonstrates that RNA extracted from herbarium specimens can be used to generate high‑quality transcriptomes, comparable to those from fresh or silica‑dried samples. By assembling and comparing transcriptomes across specimen types, the authors validated a plant immune receptor synthesized from a 1956 collection, proving archival RNA’s utility for functional genomics. These findings challenge the prevailing view that herbarium RNA is unsuitable for transcriptomic analyses.