Molecular and Phenotypic Characterization of Telomere Repeat Binding (TRBs) Proteins in Moss: Evolutionary and Functional Perspectives
Authors: Kusova, A., Hola, M., Goffova Petrova, I., Rudolf, J., Zachova, D., Skalak, J., Hejatko, J., Klodova, B., Prerovska, T., Lycka, M., Sykorova, E., Bertrand, Y. J. K., Fajkus, J., Honys, D., Prochazkova Schrumpfova, P.
The study characterizes telomere repeat binding (TRB) proteins in the model moss Physcomitrium patens, demonstrating that individual PpTRB genes are essential for normal protonemal and gametophore development and that loss of TRBs leads to telomere shortening, mirroring findings in seed plants. Transcriptome analysis of TRB mutants shows altered expression of genes linked to transcription regulation and stimulus response, while subcellular localization confirms nuclear residence and mutual interaction of PpTRBs, underscoring their conserved role in telomere maintenance across land plants.
The study characterizes the distinct and overlapping roles of the rice PI paralogs OsMADS2 and OsMADS4 in lodicule specification, flowering time, and floral organ development by analyzing null and double mutants and overexpression lines. Genome-wide binding (ChIP‑seq) and transcriptome (RNA‑seq) analyses identified downstream targets involved in cell division, cell wall remodeling, and osmotic regulation that underpin the observed phenotypes. These findings reveal novel functions for PI paralogs in reproductive development and highlight mechanisms of transcription factor diversification in Oryza sativa.