Transcriptomic analysis of genotypes derived from Rosa wichurana unveils molecular mechanisms associated with quantitative resistance to Diplocarpon rosae
The study investigated the molecular basis of quantitative resistance to black spot disease in a Rosa wichurana × Rosa chinensis F1 population, identifying two major QTLs (B3 on LG3 and B5 on LG5). RNA‑seq of inoculated and control leaf samples at 0, 3, and 5 days post‑inoculation revealed extensive transcriptional reprogramming, with QTL B3 triggering classic defense pathways and QTL B5 showing a limited, distinct response. These findings highlight complex, QTL‑specific regulation underlying durable black‑spot resistance in roses.
Molecular and Phenotypic Characterization of Telomere Repeat Binding (TRBs) Proteins in Moss: Evolutionary and Functional Perspectives
Authors: Kusova, A., Hola, M., Goffova Petrova, I., Rudolf, J., Zachova, D., Skalak, J., Hejatko, J., Klodova, B., Prerovska, T., Lycka, M., Sykorova, E., Bertrand, Y. J. K., Fajkus, J., Honys, D., Prochazkova Schrumpfova, P.
The study characterizes telomere repeat binding (TRB) proteins in the model moss Physcomitrium patens, demonstrating that individual PpTRB genes are essential for normal protonemal and gametophore development and that loss of TRBs leads to telomere shortening, mirroring findings in seed plants. Transcriptome analysis of TRB mutants shows altered expression of genes linked to transcription regulation and stimulus response, while subcellular localization confirms nuclear residence and mutual interaction of PpTRBs, underscoring their conserved role in telomere maintenance across land plants.
The study characterizes the distinct and overlapping roles of the rice PI paralogs OsMADS2 and OsMADS4 in lodicule specification, flowering time, and floral organ development by analyzing null and double mutants and overexpression lines. Genome-wide binding (ChIP‑seq) and transcriptome (RNA‑seq) analyses identified downstream targets involved in cell division, cell wall remodeling, and osmotic regulation that underpin the observed phenotypes. These findings reveal novel functions for PI paralogs in reproductive development and highlight mechanisms of transcription factor diversification in Oryza sativa.
The study identified key transcription factors governing the development of T-shaped non-glandular trichomes (TSTs) in Artemisia argyi by comparing RNA‑seq profiles of TST‑bearing and non‑TST tissue, highlighting the AarMIXTA gene family as central regulators. Overexpressing AarMIXTA1.2 in transgenic Arabidopsis thaliana markedly increased TST density, suggesting a positive regulatory role of these MIXTA genes in trichome formation and providing insights for high‑yield moxa floss cultivation.