The study characterizes the distinct and overlapping roles of the rice PI paralogs OsMADS2 and OsMADS4 in lodicule specification, flowering time, and floral organ development by analyzing null and double mutants and overexpression lines. Genome-wide binding (ChIP‑seq) and transcriptome (RNA‑seq) analyses identified downstream targets involved in cell division, cell wall remodeling, and osmotic regulation that underpin the observed phenotypes. These findings reveal novel functions for PI paralogs in reproductive development and highlight mechanisms of transcription factor diversification in Oryza sativa.

The study performed a comprehensive computational analysis of the Arabidopsis thaliana proteome, classifying 48,359 proteins by melting temperature (Tm) and melting temperature index (TI) and linking thermal stability to amino acid composition, molecular mass, and codon usage. Machine‑learning and evolutionary analyses revealed that higher molecular mass and specific codon pairs correlate with higher Tm, and that gene duplication has driven the evolution of high‑Tm proteins, suggesting a genomic basis for stress resilience.