The authors used a bottom‑up thermodynamic modelling framework to investigate how plants decode calcium signals, starting from Ca2+ binding to EF‑hand proteins and extending to higher‑order decoding modules. They identified six universal Ca2+-decoding modules that can explain variations in calcium sensitivity among kinases and provide a theoretical basis for interpreting calcium signal amplitude and frequency in plant cells.

The study examines how ectopic accumulation of methionine in Arabidopsis thaliana leaves, driven by a deregulated AtCGS transgene under a seed‑specific promoter, reshapes metabolism, gene expression, and DNA methylation. High‑methionine lines exhibit increased amino acids and sugars, activation of stress‑hormone pathways, and reduced expression of DNA methyltransferases, while low‑methionine lines show heightened non‑CG methylation without major transcriptional changes. Integrated transcriptomic and methylomic analyses reveal a feedback loop linking sulfur‑carbon metabolism, stress adaptation, and epigenetic regulation.

The study introduces a native‑condition method combining cell fractionation and immuno‑isolation to purify autophagic compartments from Arabidopsis, followed by proteomic and lipidomic characterisation of the isolated phagophore membranes. Proteomic profiling identified candidate proteins linked to autophagy, membrane remodeling, vesicular trafficking and lipid metabolism, while lipidomics revealed a predominance of glycerophospholipids, especially phosphatidylcholine and phosphatidylglycerol, defining the unique composition of plant phagophores.

The study used transcriptomic and lipidomic profiling to investigate how chia (Salvia hispanica) leaves respond to short‑term (3 h) and prolonged (27 h) heat stress at 38 °C, revealing rapid activation of calcium‑signaling and heat‑shock pathways and reversible changes in triacylglycerol levels. Nearly all heat‑responsive genes returned to baseline expression after 24 h recovery, highlighting robust thermotolerance mechanisms that could inform improvement of other oilseed crops.

The study characterizes the protein and lipid composition of chloroplast plastoglobules in the B73 maize line during a water-deficit and recovery time course, identifying key polar and neutral lipids and abundant fibrillin proteins. Quantitative proteomics revealed a strong association between Fibrillin 4 and plastoquinone‑9, suggesting a role in redox and prenyl‑lipid metabolism, thereby establishing a foundation for leveraging plastoglobules to enhance crop drought resilience.

The study shows that the SnRK1 catalytic subunit KIN10 directs tissue-specific growth‑defense programs in Arabidopsis thaliana by reshaping transcriptomes. kin10 knockout mutants exhibit altered root transcription, reduced root growth, and weakened defense against Pseudomonas syringae, whereas KIN10 overexpression activates shoot defense pathways, increasing ROS and salicylic acid signaling at the cost of growth.

The study examined how heat stress alters lipid droplet (LD) number and composition in Arabidopsis thaliana roots, revealing degradation of membrane lipids and accumulation of TAGs and LDs. Proteomic and lipidomic analyses of LDs from a specific Arabidopsis mutant identified novel LD-associated proteins, including triterpene biosynthetic enzymes, whose substrates and products also accumulate in LDs, indicating LDs function as both sinks and sources during stress‑induced membrane remodeling and specialized metabolism.

The study demonstrates that knock‑down of CDP‑DAG synthase (CDS) genes in Arabidopsis thaliana enhances resistance to multiple pathogens but incurs a growth penalty, associated with elevated reactive oxygen species and MAPK activation. Integrated lipidomics, transcriptomics, and metabolomics reveal PA accumulation in cds mutants, which triggers JA and SA signaling and increases defense‑related metabolites such as serotonin.