The study introduced full-length SOC1 genes from maize and soybean, and a partial SOC1 gene from blueberry, into tomato plants under constitutive promoters. While VcSOC1K and ZmSOC1 accelerated flowering, all three transgenes increased fruit number per plant mainly by promoting branching, and transcriptomic profiling revealed alterations in flowering, growth, and stress‑response pathways.
The study used Azo‑solubilized microsomal extraction combined with iTRAQ proteomics to identify membrane proteins whose abundance is reduced in phosphate‑limited roots of the Arabidopsis cnih5 mutant, revealing several transporters including PHT1s. Interaction of AtCNIH5 with these cargoes was confirmed via yeast split‑ubiquitin and plant tripartite split‑GFP assays, showing that AtCNIH5 operates as a low‑Pi‑responsive ER export hub that enhances growth under phosphate deficiency.
Transcriptome responses of two Halophila stipulacea seagrass populations from pristine and impacted habitats, to single and combined thermal and excess nutrient stressors, reveal local adaptive features and core stress-response genes
Authors: Nguyen, H. M., Yaakov, B., Beca-Carretero, P., Procaccini, G., Wang, G., Dassanayake, M., Winters, G., Barak, S.
The study examined transcriptomic responses of the tropical seagrass Halophila stipulacea from a pristine and an impacted site under single and combined thermal and excess nutrient stress in mesocosms. Combined stress caused greater gene reprogramming than individual stresses, with thermal effects dominating and the impacted population showing reduced plasticity but higher resilience. Core stress‑response genes were identified as potential early field indicators of environmental stress.