The study identifies the serine/threonine protein kinase CIPK14/SNRK3.15 as a regulator of sulfate‑deficiency responses in Arabidopsis thaliana seedlings, with mutants showing diminished early adaptive and later salvage responses under sulfur starvation. While snrk3.15 mutants exhibit no obvious phenotype under sufficient sulfur, the work provides a novel proteomic dataset comparing wild‑type and mutant seedlings under sulfur limitation.
The study examined how white lupin (Lupinus albus) cotyledons mobilize nitrogen and minerals during early seedling growth under nitrogen‑deficient conditions, revealing that 60 % of stored proteins degrade within eight days and are redirected to support development. Proteomic analyses showed dynamic shifts in nutrient transport, amino acid metabolism, and stress responses, and premature cotyledon removal markedly impaired growth, highlighting the cotyledon's essential role in nutrient supply and transient photosynthetic activity.
The study characterizes the protein composition of extracellular vesicles (EVs) secreted by the oomycete Phytophthora infestans, revealing enrichment of transmembrane proteins and RxLR effectors, while EV-independent secretions are dominated by cell wall–modifying enzymes. Two MARVEL‑domain proteins, PiMDP1 and PiMDP2, are identified as EV-associated markers that co‑localize with RxLR effectors, with PiMDP2 specifically accumulating at the haustorial interface during early infection, suggesting a role in effector delivery.
Whats left from the brew? Investigating residual barley proteins in spent grains for downstream valorization opportunities
Authors: Gregersen Echers, S., Mikkelsen, R. K., Abdul-Khalek, N., Queiroz, L. S., Hobley, T. J., Schulz, B. L., Overgaard, M. T., Jacobsen, C., Yesiltas, B.
The study provides an in‑depth proteomic characterization of brewer's spent grain (BSG) and tracks proteome dynamics during malting and mashing, revealing that 29% of identified proteins change in abundance and that B3‑Hordein dominates the BSG protein pool. BSG contains a high proportion of intracellular proteins and over 45% of its proteins are potential allergens or antinutritional factors, underscoring the need for targeted downstream processing to create safe, functional food ingredients.
The study profiled the Arabidopsis apoplastic proteome during pattern‑triggered immunity induced by the flg22 peptide, using apoplastic washing fluid with minimal cytoplasmic contamination followed by LC‑MS/MS. Results showed consistent PTI‑specific enrichment and depletion of peptides, a bias toward ectodomain peptides of receptor‑like kinases, and increased abundance of the exosome marker tetraspanin 8, indicating heightened exosome levels during PTI.
Transcriptome responses of two Halophila stipulacea seagrass populations from pristine and impacted habitats, to single and combined thermal and excess nutrient stressors, reveal local adaptive features and core stress-response genes
Authors: Nguyen, H. M., Yaakov, B., Beca-Carretero, P., Procaccini, G., Wang, G., Dassanayake, M., Winters, G., Barak, S.
The study examined transcriptomic responses of the tropical seagrass Halophila stipulacea from a pristine and an impacted site under single and combined thermal and excess nutrient stress in mesocosms. Combined stress caused greater gene reprogramming than individual stresses, with thermal effects dominating and the impacted population showing reduced plasticity but higher resilience. Core stress‑response genes were identified as potential early field indicators of environmental stress.