The study functionally characterizes three tomato CNR/FWL proteins (SlFWL2, SlFWL4, SlFWL5) and demonstrates that SlFWL5 localizes to plasmodesmata, where it regulates leaf size and morphology by promoting cell expansion likely through cell‑to‑cell communication. Gain‑ and loss‑of‑function transgenic tomato lines reveal that SlFWL5 is a key regulator of organ growth via modulation of plasmodesmatal signaling.
The study constructs a ~1‑million‑cell single‑nuclei transcriptome atlas of Arabidopsis leaves to reveal that drought stress accelerates transcriptional programs associated with maturation and aging, thereby limiting leaf growth in proportion to stress intensity. Targeted upregulation of FERRIC REDUCTION OXIDASE 6 in mesophyll cells partially rescues leaf growth under drought, demonstrating the functional relevance of these transcriptional changes.
RNA‑seq of 328 wheat lines using a pan‑genome reference uncovered over 20,000 additional transcripts beyond the Chinese Spring genome and enabled construction of a pan‑gene eQTL regulatory atlas. Multi‑omics integration identified 231 high‑confidence candidate genes influencing 34 agronomic traits and powdery mildew resistance, with functional validation showing 80% of candidates affecting trait phenotypes via an EMS mutant library.
The study investigates how miR394 influences flowering time in Arabidopsis thaliana by combining transcriptomic profiling of mir394a mir394b double mutants with histological analysis of reporter lines. Bioinformatic analysis identified a novel lncRNA overlapping MIR394B (named MIRAST), and differential promoter activity of MIR394A and MIR394B suggests miR394 fine‑tunes flower development through transcription factor and chromatin remodeler regulation.
This review compiles experimental studies on wheat to assess how elevated CO₂, higher temperatures, and water deficit interact and affect productivity and water use. By calculating plasticity indices, the authors find that despite CO₂‑induced gains, overall yield generally declines under combined stress, while water consumption often decreases. They highlight the need for more data to improve and validate crop models under future climate scenarios.
The study introduces Transposase-Accessible Chromosome Conformation Capture (TAC-C), which combines ATAC‑seq and Hi‑C to map fine‑scale chromatin interactions in rice, sorghum, maize, and wheat, revealing genome‑size‑correlated loop structures and distinct C3 vs. C4 patterns. Integration with population genetics shows that loops link distal regulatory elements to phenotypic variation, and SPL transcription factors (TaSPL7/15) modulate photosynthesis‑related genes via these interactions, enhancing photosynthetic efficiency and starch content in wheat mutants.