The study examined molecular responses in grapevine leaves with and without esca symptoms, using metabolite profiling, RNA‑seq and whole‑genome bisulfite sequencing. Metabolic and transcriptomic changes were confined to symptomatic leaves and linked to local DNA‑methylation alterations, while asymptomatic leaves showed distinct but overlapping methylation patterns, some present before symptoms, indicating potential epigenetic biomarkers for early disease detection.
Stress-dependent responses of grapevine wood and fungal pathogen activity under esca and drought
Authors: Chambard, M., Cantu, D., Bortolami, G., Dell'Acqua, N., Ferrer, N., Gambetta, G., Garcia, J., Gastou, P., Massonnet, M., Moretti, S., Rochepeau, A., Petriacq, P., Foulongne-Oriol, M., Delmas, C. E. L.
The study used wood metatranscriptomics, metabolomics, and metabarcoding to compare grapevine (Vitis vinifera) responses to drought and esca leaf symptom expression, revealing distinct but overlapping transcriptomic and metabolic signatures, including activation of phenylpropanoid and stilbenoid pathways. Drought reduced esca symptom expression, associated with decreased abundance of the wood‑decay fungus Fomitiporia mediterranea and altered fungal virulence factor expression, while increasing the relative abundance and anti‑oxidative gene expression of Phaeomoniella chlamydospora.