The study maps the in vivo proximity interactome of Arabidopsis SKP1-LIKE 1 (ASK1) under acute abscisic acid (ABA) signaling and prolonged drought using TurboID-based proximity labeling and quantitative proteomics, revealing condition-specific networks that include both canonical SCF modules and diverse noncanonical partners. Overexpression of ASK1 shifts proteome composition toward drought‑protective and ABA‑responsive proteins while repressing immune and ROS‑scavenging pathways, highlighting ASK1 as a hub that integrates SCF‑dependent and independent pathways to reprogram transcription, translation, and proteostasis during stress adaptation.
The study reveals that REMORIN protein evolution is primarily driven by diversification of their conserved C-terminal domain, defining four major clades. Structural bioinformatics predicts a common membrane‑binding interface with diverse curvatures and lengths, and suggests that some REMs can form C‑terminal‑mediated oligomers, adding complexity to membrane organization.
Sixteen upland rice varieties were evaluated under three irrigation regimes (100%, 70%, and 50% field capacity) with additional six‑day water withholding to simulate moderate and severe drought. Yield losses ranged from 35% to 78% depending on stress level, and varieties Dawk Kha, Khao/Sai, and Dawk Pa‑yawm showed the greatest stability, suggesting they are promising for breeding drought‑resilient upland rice.
The study identified a heat‑responsive exon‑skipping event in the basic Helix‑Loop‑Helix domain of the transcription factor PIF4, which reduces PIF4 activity and promotes photomorphogenic traits in etiolated seedlings. This reveals a novel post‑transcriptional mechanism by which plants modulate PIF4 function during heat stress.
The study evaluated how stomatal anatomy and physiological efficiency influence wheat heat tolerance across multi‑environment field trials with 200 genotypes, using early versus delayed sowing to impose temperature stress. Findings revealed a decoupling between anatomical capacity (gsmax) and actual conductance (gs, gse) under heat, plastic shifts toward smaller, denser stomata, and identified 125 QTL linked to stomatal traits, suggesting targets for breeding climate‑resilient wheat.
The study examined how DNA methylation influences cold stress priming in Arabidopsis thaliana, revealing that primed plants exhibit distinct gene expression and methylation patterns compared to non-primed plants. DNA methylation mutants, especially met1 lacking CG methylation, showed altered cold memory and misregulation of the CBF gene cluster, indicating that methylation ensures transcriptional precision during stress recall.
Quantitative trait locus mapping of root exudate metabolome in a Solanum lycopersicum Moneymaker x S. pimpinellifolium RIL population and their putative links to rhizosphere microbiome
Authors: Kim, B., Kramer, G., Leite, M. F. A., Snoek, B. L., Zancarini, A., Bouwmeester, H.
The study used untargeted metabolomics and QTL mapping in a tomato recombinant inbred line population to characterize root exudate composition and identify genetic loci controlling specific metabolites. It reveals domestication-driven changes in exudate profiles and links metabolic QTLs with previously reported microbial QTLs, suggesting a genetic basis for shaping the root microbiome.
The study identified a critical two‑week window of elevated maternal temperature during weeks 4–5 after flowering that delays dormancy release in weedy rice seeds. Controlled‑environment and field transplant experiments showed that this late‑reproductive‑stage heat exposure postpones germination after after‑ripening, providing insight for predicting seed behavior and improving weed management strategies.
The study used Weighted Gene Correlation Network Analysis (WGCNA) and GENIE3 to construct co‑expression modules and gene regulatory networks (GRNs) in barley subjected to Fusarium head blight and drought stress. Integration of these approaches highlighted overlapping regulatory patterns, pinpointing WRKY transcription factors as central to FHB response, while bHLH and NAC family members showed stress‑specific roles. Promoter motif enrichment further validated predicted TF‑target interactions, offering candidate regulators for future functional validation.
The authors used computational simulations of plant cellular metabolism under historical atmospheric conditions to demonstrate that reduced CO₂ and increased aridity can drive the evolutionary transition from C₃ to CAM photosynthesis. Their results suggest that while future elevated CO₂ may favor a reversion to C₃-like behavior, drought consistently promotes CAM regardless of CO₂ or temperature, and a minimum O₂ level is required for nocturnal respiration in CAM.