Genetius

The authors introduce the ENABLE(R) Gene Editing in planta toolkit, a streamlined two‑step cloning system for creating CRISPR/Cas9 knockout vectors suitable for transient or stable transformation. Validation was performed in Oryza sativa protoplasts and Arabidopsis thaliana plants, and the toolkit includes low‑cost protocols aimed at facilitating adoption in the Global South.

The study mapped drought‑responsive gene regulatory networks in Arabidopsis thaliana, its tolerant relative Arabidopsis lyrata, and Eutrema salsugineum using yeast one‑hybrid screens of orthologous promoters, revealing higher network connectivity and specific TF‑promoter interactions in the tolerant species. Notable findings include an Esa‑specific expansion of bZIP interactions, differential ABA‑signalling edges, and the identification of ASIL2 as a novel stress‑responsive factor, providing a comparative framework for improving crop drought tolerance.

The study finely dissected shoot stem cell–enriched tissues from maize and Arabidopsis thaliana and optimized single‑cell RNA‑seq protocols to reliably capture CLAVATA3 and WUSCHEL‑expressing cells. Cross‑species comparison and functional validation, including spatial transcriptomics and mutant analyses, revealed conserved ribosome‑associated RNA‑binding proteins and sugar‑kinase families as key regulators linked to shoot development and yield traits.

The study developed a validated LC‑MS/MS method to simultaneously quantify fourteen polyamines, amino acids, and ethylene precursors in Arabidopsis thaliana and Solanum lycopersicum, and used it to compare their metabolic responses to drought, salinity, and inhibitor treatments. Distinct species‑specific metabolic adjustments were observed, with Arabidopsis showing greater fluctuations and drought generally increasing metabolite levels, while spermine exhibited stress‑specific patterns.