The study maps the in vivo proximity interactome of Arabidopsis SKP1-LIKE 1 (ASK1) under acute abscisic acid (ABA) signaling and prolonged drought using TurboID-based proximity labeling and quantitative proteomics, revealing condition-specific networks that include both canonical SCF modules and diverse noncanonical partners. Overexpression of ASK1 shifts proteome composition toward drought‑protective and ABA‑responsive proteins while repressing immune and ROS‑scavenging pathways, highlighting ASK1 as a hub that integrates SCF‑dependent and independent pathways to reprogram transcription, translation, and proteostasis during stress adaptation.
Sixteen upland rice varieties were evaluated under three irrigation regimes (100%, 70%, and 50% field capacity) with additional six‑day water withholding to simulate moderate and severe drought. Yield losses ranged from 35% to 78% depending on stress level, and varieties Dawk Kha, Khao/Sai, and Dawk Pa‑yawm showed the greatest stability, suggesting they are promising for breeding drought‑resilient upland rice.
The study identified a heat‑responsive exon‑skipping event in the basic Helix‑Loop‑Helix domain of the transcription factor PIF4, which reduces PIF4 activity and promotes photomorphogenic traits in etiolated seedlings. This reveals a novel post‑transcriptional mechanism by which plants modulate PIF4 function during heat stress.
The study evaluated how stomatal anatomy and physiological efficiency influence wheat heat tolerance across multi‑environment field trials with 200 genotypes, using early versus delayed sowing to impose temperature stress. Findings revealed a decoupling between anatomical capacity (gsmax) and actual conductance (gs, gse) under heat, plastic shifts toward smaller, denser stomata, and identified 125 QTL linked to stomatal traits, suggesting targets for breeding climate‑resilient wheat.
The study identifies MtFTb1 and MtFTb2 as essential, redundant regulators of long‑day flowering in the legume Medicago truncatula, demonstrating that they are required for up‑regulating MtFTa1 under vernalised long‑day conditions. Using CRISPR/Cas9‑generated single and double mutants, the authors show that double mutants are specifically delayed in flowering under long days while retaining vernalization responsiveness, and transcriptomic analyses reveal that MtFTb1/2 activate MADS‑box genes and other flowering regulators.
The study identified a critical two‑week window of elevated maternal temperature during weeks 4–5 after flowering that delays dormancy release in weedy rice seeds. Controlled‑environment and field transplant experiments showed that this late‑reproductive‑stage heat exposure postpones germination after after‑ripening, providing insight for predicting seed behavior and improving weed management strategies.
The study used Weighted Gene Correlation Network Analysis (WGCNA) and GENIE3 to construct co‑expression modules and gene regulatory networks (GRNs) in barley subjected to Fusarium head blight and drought stress. Integration of these approaches highlighted overlapping regulatory patterns, pinpointing WRKY transcription factors as central to FHB response, while bHLH and NAC family members showed stress‑specific roles. Promoter motif enrichment further validated predicted TF‑target interactions, offering candidate regulators for future functional validation.
A comprehensive multi‑environment trial of 437 maize testcross hybrids derived from 38 MLN‑tolerant lines and 29 testers identified additive genetic effects as the primary driver of grain yield, disease resistance, and drought tolerance. Strong general combining ability and specific combining ability patterns were uncovered, with top hybrids delivering up to 5.75 t ha⁻¹ under MLN pressure while maintaining high performance under optimum and drought conditions. The study provides a framework for selecting elite parents and exploiting both additive and non‑additive effects to develop resilient maize hybrids for sub‑Saharan Africa.
The authors used computational simulations of plant cellular metabolism under historical atmospheric conditions to demonstrate that reduced CO₂ and increased aridity can drive the evolutionary transition from C₃ to CAM photosynthesis. Their results suggest that while future elevated CO₂ may favor a reversion to C₃-like behavior, drought consistently promotes CAM regardless of CO₂ or temperature, and a minimum O₂ level is required for nocturnal respiration in CAM.
The study presents an optimized Agrobacterium-mediated transformation toolkit for Sorghum bicolor that achieves up to 95.7% editing efficiency using CRISPR/Cas9 targeting the SbPDS gene, and demonstrates comparable performance with a PAM‑broadened SpRY variant. This platform enables multiplex genome editing and is positioned for integration of advanced tools such as prime and base editors to accelerate sorghum breeding.