Integrating physiological, transcriptomic, and cellular analyses, the study shows that olive fruit abscission zones undergo lignification, alkalization, and extensive cell‑wall remodeling during natural maturation and after ethephon treatment. A set of 733 FAZ‑specific genes, including β‑1,3‑glucanases, pectate lyases, and pH‑regulating transporters, were identified, and increased glucanase activity together with reduced plasmodesmata callose suggest enhanced intercellular communication facilitates organ detachment in this non‑climacteric fruit.

The study investigates the molecular basis of heat‑tolerant pollen tube growth in tomato (Solanum lycopersicum) by comparing thermotolerant and sensitive cultivars. Using live imaging, transcriptomics, proteomics, and genetics, the authors identified the Rapid Alkalinization Factor (RALF) signaling pathway as a key regulator of pollen tube integrity under high temperature, with loss of a specific RALF peptide enhancing tube integrity in a thermotolerant cultivar.

The study reveals that the Arabidopsis O-GlcNAc transferase SEC is essential for timely ABA‑induced stomatal closure and drought tolerance, with sec-5 mutants showing delayed closure and increased water loss, while SEC overexpression enhances responsiveness. SEC influences guard‑cell microtubule remodeling, as loss of SEC impairs microtubule reorganization and SEC directly interacts with tubulin α‑4, suggesting tubulin as a target of O‑GlcNAcylation.

The study examined drought tolerance across nine provenances of the conifer Pinus nigra using high‑throughput phenotyping combined with metabolomic and transcriptomic analyses under controlled soil‑drying conditions. Drought tolerance, measured by the decline in Fv/Fm, varied among provenances but was not linked to a climatic gradient and was independent of growth, with tolerant provenances showing distinct flavonoid and diterpene profiles and provenance‑specific gene expression patterns. Integrating phenotypic and molecular data revealed metabolic signatures underlying drought adaptation in this non‑model conifer.

The study applied limited proteolysis‑coupled mass spectrometry (LiP‑MS) to map JA‑protein interactions, validating known JA binders and uncovering novel candidates, including several UDP‑glucuronosyltransferases (UGTs). Functional omics, biochemical, enzymatic, and structural analyses demonstrated that two tomato UGTs glucosylate jasmonic acid, revealing a previously missing step in JA catabolism.

The study demonstrates that limonene, a natural essential‑oil component, strongly inhibits Fusarium oxysporum, the causal agent of potato dry rot, by impairing colony growth, hyphal morphology, spore viability, membrane integrity, and transcription/translation processes, as well as disrupting ion homeostasis. Combined treatments reveal additive effects with mancozeb and synergistic effects with hymexazol, highlighting limonene's potential as an eco‑friendly bio‑fungicide for potato disease management.

The study compared physiological and transcriptomic responses of poplar trees colonized by the ectomycorrhizal fungi Paxillus involutus or Cenococcum geophilum under normal, drought, and recovery conditions. Cenococcum-colonized plants showed constitutive up‑regulation of heat‑shock proteins, galactinol synthase, and aquaporins and maintained water status and photosynthesis during severe drought, whereas Paxillus colonization promoted growth and nitrogen‑use efficiency and enabled rapid recovery through drought‑induced leaf shedding. These contrasting strategies illustrate species‑specific positions on the growth‑defense trade‑off in ectomycorrhizal symbiosis.

The study assessed the impact of adding mammalian growth factors and cytokines to transformation media on CRISPR‑Cas9–mediated genome editing in six tomato (Solanum lycopersicum) accessions with varying regeneration capacities. Over three years, supplementation with these factors significantly increased regeneration rates and the production of stable secondary transgenic lines, especially in recalcitrant genotypes.

The researchers created tomato lines overexpressing the autophagy gene SlATG8f and evaluated their performance under high-temperature stress. qRT‑PCR and physiological measurements revealed that SlATG8f overexpression enhances expression of autophagy‑related and heat‑shock protein genes, accelerates fruit ripening, and improves fruit quality under heat stress.

Proteomic comparison of mock‑ and potato spindle tuber viroid‑infected tomato revealed a broad down‑regulation of nucleoporins and nuclear transport receptors, leading to impaired nuclear import of the immune regulator NPR1. Overexpression of NPR1 or treatment with a salicylic‑acid analog restored defense and reduced PSTVd infection, highlighting nuclear transport repression as a key vulnerability in plant immunity against viroids.