The study compares transcriptional, proteomic, and metabolomic responses of wild‑type Arabidopsis and a cyp71A27 mutant to a plant‑growth‑promoting Pseudomonas fluorescens strain and a pathogenic Burkholderia glumeae strain, revealing distinct reprogramming and an unexpected signaling role for the non‑canonical P450 CYP71A27. Mutant analysis showed that loss of CYP71A27 alters gene and protein regulation, especially during interaction with the PGP bacterium, while having limited impact on root metabolites and exudates.

The study generated wheat (Triticum aestivum) mutants with targeted deletions in the SAL gene family (TaSAL1 and TaSAL2) to assess the impact of chloroplast-to-nucleus retrograde signaling on field performance. Across 15 diverse Australian field trials, TaSAL2 deletions conferred 4–8% higher yields and improved water productivity by maintaining photosynthetic efficiency and dynamic stomatal control under drought, whereas TaSAL1 deletions reduced yields. These results demonstrate that locus‑specific retrograde signaling modifications can simultaneously enhance yield and stress resilience in a major crop.

The study presents a chromosome-level reference genome for the invasive fern Lygodium microphyllum and compares the transcriptomic and epigenomic profiles of its haploid gametophyte and diploid sporophyte phases, revealing differential regulation of developmental genes and similar methylation patterns across tissues. Base‑pair resolution methylome data and freezing‑stress experiments show that each life phase employs distinct molecular pathways for stress response, emphasizing the importance of considering both phases in invasive‑species management.

The study investigates how miR394 influences flowering time in Arabidopsis thaliana by combining transcriptomic profiling of mir394a mir394b double mutants with histological analysis of reporter lines. Bioinformatic analysis identified a novel lncRNA overlapping MIR394B (named MIRAST), and differential promoter activity of MIR394A and MIR394B suggests miR394 fine‑tunes flower development through transcription factor and chromatin remodeler regulation.

The study evaluated high‑throughput spectroscopy and poro‑fluorometry to predict leaf morphological and ecophysiological traits in a grapevine diversity panel under well‑watered and drought conditions. Spectroscopy reliably estimated leaf mass per area and water content, while poro‑fluorometry accurately predicted net CO2 assimilation, and the derived predicted traits showed substantial broad‑sense heritability. These results demonstrate that non‑destructive, rapid phenotyping tools can support genetic analyses of drought‑related traits in grapevine.