Genetius

The study shows that inoculating Arabidopsis thaliana with the plant‑growth‑promoting bacterium Enterobacter sp. SA187 markedly boosts root and shoot biomass under elevated CO₂, accompanied by altered nitrogen and carbon content and reshaped phytohormone signaling. Transcriptomic and metabolomic analyses reveal activation of salicylic acid, jasmonic acid, and ethylene pathways and enhanced primary metabolism, while the ethylene‑insensitive ein2‑1 mutant demonstrates that the growth benefits are ethylene‑dependent.

The study investigated the five Arabidopsis SCAMP proteins, focusing on SCAMP5, and identified conserved tyrosine and NPF motifs that mediate anterograde transport and endocytosis, respectively. SCAMPs were shown to dimerize at the plasma membrane and endosomes, interact with plasma‑membrane aquaporins, and their loss (triple and quintuple mutants) conferred mild developmental delay but increased drought tolerance, likely via altered PIP trafficking or stability.

The study combined cell biology, transcriptomics, and ionomics to reveal that zinc deficiency reduces root apical meristem size while preserving meristematic activity and local Zn levels, leading to enhanced cell elongation and differentiation in Arabidopsis thaliana. ZIP12 was identified as a highly induced gene in the zinc‑deficient root tip, and zip12 mutants displayed impaired root growth, altered RAM structure, disrupted Zn‑responsive gene expression, and abnormal metal partitioning, highlighting ZIP12’s role in maintaining Zn homeostasis and meristem function.

The study uses a high‑throughput comparative multi‑omics strategy to profile transcript, metabolite, and protein dynamics in Arabidopsis thaliana seedlings throughout the heat‑stress memory (HSM) phase following acquired thermotolerance. Early recovery stages show rapid transcriptional activation of memory‑related genes, while protein levels stay elevated longer, and distinct metabolite patterns emerge, highlighting temporal layers of the memory process.

The study compared two plasma‑activated water (PAW) solutions with different H₂O₂ levels, produced by a radio‑frequency glow discharge, on Arabidopsis thaliana growth and stress responses. PAW lacking detectable H₂O₂ promoted seedling growth and induced nitrogen‑assimilation genes, while H₂O₂‑containing PAW did not affect growth but enhanced root performance under heat stress; mature plants fertilized with H₂O₂‑free PAW performed comparably to nitrate controls. These results indicate PAW can replace NO₃⁻ fertilizers provided H₂O₂ levels are carefully managed.

The study introduces a CRISPR/Cas9‑based restoration system (CiRBS) that reactivates a disabled luciferase reporter (LUC40Ins26bp) in transgenic Arabidopsis, enabling long‑term single‑cell bioluminescence monitoring. Restoration occurs within 24 h after particle‑bombardment‑mediated CRISPR delivery, with ~7 % of cells regaining luminescence and most restored cells carrying a single correctly edited chromosome, facilitating reliable analysis of cellular gene‑expression heterogeneity.

The study demonstrates that N6‑methyladenosine (m6A) RNA methylation acts as a negative regulator of thermotolerance in Arabidopsis thaliana, with loss of m6A increasing heat‑responsive gene expression and mRNA stability. Heat shock triggers a transient reduction of m6A levels, which is linked to enrichment of the H3K4me3 histone mark at target loci, enhancing transcription of heat shock proteins. These findings reveal a coordinated interplay between RNA methylation and chromatin modifications that fine‑tunes the plant heat stress response.

The study used Arabidopsis thaliana mutants with low (vtc2, vtc4) and high (vtc2/OE-VTC2) ascorbate levels to examine how ascorbate concentration affects gene expression and cellular homeostasis. Transcriptomic analysis revealed that altered ascorbate levels modulate defense and stress pathways, and that TAA1/TAR2‑mediated auxin biosynthesis is required for coping with elevated ascorbate in a light‑dependent manner.

The study examined how Arabidopsis thaliana integrates physiological, genetic, hormonal, and transcriptomic responses to combined water deficit, heat stress, and elevated CO2. Results show that stomatal aperture under these complex stress combinations is governed by a specific set of regulators, including nitric oxide, OPEN STOMATA 1, and the SLAH3 anion channel, distinct from those active under simpler stress conditions. This reveals a hierarchical stomatal stress code that could inform future research on plant resilience to global change.

Loss‑of‑function mutations in the drought‑induced genes GASA3 and AFP1 confer enhanced drought tolerance in Arabidopsis thaliana, primarily through smaller stomatal apertures and increased ABA accumulation via hydrolysis of ABA‑GE. Constitutive overexpression of these genes heightens drought sensitivity, indicating that the AFP1/GASA3 module negatively regulates stomatal closure and ABA signaling.