A novel pathosystem between Aeschynomene evenia and Aphanomyces euteiches reveals new immune components in quantitative legume root-rot resistance.
Authors: Baker, M., Martinez, Y., Keller, J., Sarrette, B., Pervent, M., Libourel, C., Le Ru, A., Bonhomme, M., Gough, C., Castel, B., ARRIGHI, J.-F., Jacquet, C.
The study establishes Aeschynomene evenia as a new model for dissecting legume immunity against the soilborne pathogen Aphanomyces euteiches and its relationship with Nod factor-independent symbiosis. Quantitative resistance was assessed through inoculation assays, phenotypic and cytological analyses, and RNA‑seq identified thousands of differentially expressed genes, highlighting immune signaling and specialized metabolism, with mutant analysis confirming dual‑function kinases that modulate resistance. Comparative transcriptomics with Medicago truncatula revealed conserved and unique immune responses, positioning the A. evenia–A. euteiches system as a valuable platform for exploring quantitative resistance and symbiosis integration.
The authors used a bottom‑up thermodynamic modelling framework to investigate how plants decode calcium signals, starting from Ca2+ binding to EF‑hand proteins and extending to higher‑order decoding modules. They identified six universal Ca2+-decoding modules that can explain variations in calcium sensitivity among kinases and provide a theoretical basis for interpreting calcium signal amplitude and frequency in plant cells.