The study used transcriptomic profiling to compare tomato (Solanum lycopersicum) responses to three evolutionarily distant pathogens—nematodes, aphids, and oomycetes—during compatible interactions, identifying differentially expressed genes and key host hubs. Integrating public datasets and performing co‑expression and GO enrichment analyses, the authors mapped shared dysregulation clusters and employed Arabidopsis interactome data to place tomato candidates within broader networks, highlighting potential targets for multi‑pathogen resistance.

The study examined how polyploidy, hybridization, and incomplete lineage sorting affect phylogenetic reconstructions in the genus Packera, evaluating several published paralog‑processing pipelines. Results showed that the choice of orthology and paralog handling methods markedly altered tree topology, time‑calibrated phylogenies, biogeographic histories, and detection of ancient reticulation, underscoring the need for careful methodological selection alongside comprehensive taxon sampling.

A long‑term FACE experiment exposing 180‑year‑old Quercus robur to +150 ppm CO₂ showed seasonal declines in powdery mildew and insect herbivory but no consistent change in biotic stress incidence. Metabolomic analyses revealed widespread shifts in amino acid, Coenzyme A, and redox pathways, indicating extensive metabolic plasticity without altered resistance to pathogens or herbivores.