The study compares transcriptional, proteomic, and metabolomic responses of wild‑type Arabidopsis and a cyp71A27 mutant to a plant‑growth‑promoting Pseudomonas fluorescens strain and a pathogenic Burkholderia glumeae strain, revealing distinct reprogramming and an unexpected signaling role for the non‑canonical P450 CYP71A27. Mutant analysis showed that loss of CYP71A27 alters gene and protein regulation, especially during interaction with the PGP bacterium, while having limited impact on root metabolites and exudates.

The study examined how heat stress alters lipid droplet (LD) number and composition in Arabidopsis thaliana roots, revealing degradation of membrane lipids and accumulation of TAGs and LDs. Proteomic and lipidomic analyses of LDs from a specific Arabidopsis mutant identified novel LD-associated proteins, including triterpene biosynthetic enzymes, whose substrates and products also accumulate in LDs, indicating LDs function as both sinks and sources during stress‑induced membrane remodeling and specialized metabolism.

The study presents a chromosome-level reference genome for the invasive fern Lygodium microphyllum and compares the transcriptomic and epigenomic profiles of its haploid gametophyte and diploid sporophyte phases, revealing differential regulation of developmental genes and similar methylation patterns across tissues. Base‑pair resolution methylome data and freezing‑stress experiments show that each life phase employs distinct molecular pathways for stress response, emphasizing the importance of considering both phases in invasive‑species management.

The study investigates how miR394 influences flowering time in Arabidopsis thaliana by combining transcriptomic profiling of mir394a mir394b double mutants with histological analysis of reporter lines. Bioinformatic analysis identified a novel lncRNA overlapping MIR394B (named MIRAST), and differential promoter activity of MIR394A and MIR394B suggests miR394 fine‑tunes flower development through transcription factor and chromatin remodeler regulation.

The study demonstrates that knock‑down of CDP‑DAG synthase (CDS) genes in Arabidopsis thaliana enhances resistance to multiple pathogens but incurs a growth penalty, associated with elevated reactive oxygen species and MAPK activation. Integrated lipidomics, transcriptomics, and metabolomics reveal PA accumulation in cds mutants, which triggers JA and SA signaling and increases defense‑related metabolites such as serotonin.