The study examined how polyploidy, hybridization, and incomplete lineage sorting affect phylogenetic reconstructions in the genus Packera, evaluating several published paralog‑processing pipelines. Results showed that the choice of orthology and paralog handling methods markedly altered tree topology, time‑calibrated phylogenies, biogeographic histories, and detection of ancient reticulation, underscoring the need for careful methodological selection alongside comprehensive taxon sampling.

The study identifies an autophagy pathway that degrades plasma membrane-derived clathrin-coated vesicles (CCVs) during hyperosmotic stress, helping maintain membrane tension as cell volume decreases. Using live imaging and correlative microscopy, the authors show that the TPLATE complex subunits AtEH1/Pan1 and AtEH2/Pan1 act as selective autophagy receptors by directly binding ATG8, thereby removing excess membrane under drought or salt conditions.

The study investigates hormetic responses of tomato (Solanum lycopersicum) seedlings to low‑dose cadmium, demonstrating enhanced growth through morphological, biochemical, and histochemical analyses. Transcriptomic profiling revealed differential expression of oxidoreductase genes, signaling components, and several long non‑coding RNAs (lncRNAs) that generate miRNAs (sly‑MIR396a and sly‑MIR1063g), which modulate target genes to promote growth. In‑silico analyses of lncRNA targets and miRNA precursors provide mechanistic insight into cadmium‑induced hormesis and its potential for crop improvement.

The study investigates how miR394 influences flowering time in Arabidopsis thaliana by combining transcriptomic profiling of mir394a mir394b double mutants with histological analysis of reporter lines. Bioinformatic analysis identified a novel lncRNA overlapping MIR394B (named MIRAST), and differential promoter activity of MIR394A and MIR394B suggests miR394 fine‑tunes flower development through transcription factor and chromatin remodeler regulation.