Keywords: paralog processing, ascorbate, CO2 enrichment, CLPC2, Agrobacterium-mediated transformation

Latest 13 Papers

Strain, procedures, and tools for reproducible genetic transformation and genome editing of Spirodela polyrhiza (L.) Schleid.

Authors: Barragan-Borrero, V., de Santana Lopes, A., Rodrigues-Batista, E. D., Hofer, M., Elias, R., Chakraborty, A., Ponce-Mane, A., Descombes, C., Diezma-Navas, L., Petraki, L., Huber, M., Xu, S., Mari-Ordonez, A.

Date: 2025-12-14 · Version: 2

DOI: 10.1101/2025.08.28.672806

Model Organism: Spirodela polyrhiza

Spirodela polyrhiza duckweed Agrobacterium-mediated transformation CRISPR/Cas9 genome editing callus induction

Stable Inheritance of Transgene and Yellow Fluorescent Protein Gene Expression in Progeny of Transgenic Cacao (Theobroma cacao) Plants

Authors: Austin, G., Jones, J., Stevens, A., Zhang, E., Thompson, T., Gomez, M., Vrla, G., Oh, Y., Marelli, J.-P., Jones, C., Staskawicz, B., Cho, M.-J.

Date: 2025-12-09 · Version: 1

DOI: 10.64898/2025.12.09.692773

Model Organism: Theobroma cacao

cacao Agrobacterium-mediated transformation somatic embryo explants transgene inheritance fluorescent marker

High-frequency sorghum transformation toolkit enhances Cas9 efficiency and expands promoter-editing capability with SpRY

Authors: Shen, J., Aregawi, K., Anwar, S., Miller, T., Groover, E. D., Rajkumar, M., Savage, D. F., Lemaux, P. G.

Date: 2025-12-07 · Version: 2

DOI: 10.1101/2025.01.21.634149

Model Organism: Sorghum bicolor

Sorghum bicolor CRISPR/Cas9 Agrobacterium-mediated transformation SpRY (PAM‑flexible Cas9)high-efficiency genome editing

CLPC2 plays specific roles in CLP complex-mediated regulation of growth, photosynthesis, embryogenesis and response to growth-promoting microbial compounds

Authors: Leal-Lopez, J., Bahaji, A., De Diego, N., Tarkowski, P., Baroja-Fernandez, E., Munoz, F. J., Almagro, G., Perez, C. E., Bastidas-Parrado, L. A., Loperfido, D., Caporalli, E., Ezquer, I., Lopez-Serrano, L., Ferez-Gomez, A., Coca-Ruiz, V., Pulido, P., Morcillo, R. J. L., Pozueta-Romero, J.

Date: 2025-11-28 · Version: 1

DOI: 10.1101/2025.11.25.690394

Model Organism: Arabidopsis thaliana

CLPC2 microbial volatile compounds chloroplast CLP protease proteomics Arabidopsis thaliana

Binary vector origin predictably determines Agrobacterium-mediated transformation outcome across eukaryotic kingdom

Authors: Szarzanowicz, M., Busche, M., Dai, Z., Lapp, N., Geiselman, G., Jia, J., Waldburger, L., Liang, Y., Yuan, G., Duong, R., Hillson, N., Brunkard, J. O., Simmons, B. A., Gladden, J., Kim, J., Thompson, M., Shih, P.

Date: 2025-11-18 · Version: 1

DOI: 10.1101/2025.11.18.688525

Model Organism: Arabidopsis thaliana

Agrobacterium-mediated transformation origin of replication T-DNA insertion number transgene silencing binary vector design

Leveraging Crop Wild Relatives and Ploidy Level for Climate-Resilient Annual Ryegrass

Authors: Mailhos, M. E., Timmers, J., Erickson, J., Caram, N., Messina, C. D., Rios, E. F.

Date: 2025-10-18 · Version: 1

DOI: 10.1101/2025.10.15.682691

Model Organism: Lolium multiflorum

polyploidy climate change Lolium multiflorum CO2 enrichment evapotranspiration

Improving Perennial Ryegrass Transformation Protocols with Developmental Regulators

Authors: Cors, J., Olson-Jenson, G., Watkins, E., Smanski, M.

Date: 2025-10-15 · Version: 1

DOI: 10.1101/2025.10.14.682379

Model Organism: Lolium perenne

genetically modified turfgrass Agrobacterium-mediated transformation WUSCHEL2 BABY BOOM perennial ryegrass

Phylogenomic challenges in polyploid-rich lineages: Insights from paralog processing and reticulation methods using the complex genus Packera (Asteraceae: Senecioneae)

Authors: Moore-Pollard, E. R., Ellestad, P., Mandel, J.

Date: 2025-10-13 · Version: 2

DOI: 10.1101/2025.09.14.676143

Model Organism: Multi-species

phylogenomic discordance polyploidy paralog processing Packera historical biogeography

Choosing the Best Route: Comparative Optimization of Wheat Transformation Methods for Improving Yield by Targeting TaARE1-D with CRISPR/Cas9

Authors: Tek, M. I., Budak Tek, K., Sarikaya, P., Ahmed, A. R., Fidan, H.

Date: 2025-09-12 · Version: 1

DOI: 10.1101/2025.09.11.675438

Model Organism: Triticum aestivum

Triticum aestivum CRISPR/Cas9 Agrobacterium-mediated transformation TaARE1-D yield improvement

Controlling GRF4-GIF1 Expression for Efficient, Genotype-Independent Transformation Across Wheat Cultivars

Authors: Hayta, S., Smedley, M. A., Bayraktar, M., Forner, M., Backhaus, A., Lister, C., Clarke, M., Uauy, C., Griffiths, S.

Date: 2025-09-02 · Version: 1

DOI: 10.1101/2025.08.29.673042

Model Organism: Triticum aestivum

GRF4‑GIF1 fusion Agrobacterium-mediated transformation genotype‑independent regeneration bread wheat transformation efficiency

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