The authors compiled and standardized published data on Rubisco dark inhibition for 157 flowering plant species, categorizing them into four inhibition levels and analyzing phylogenetic trends. Their meta‑analysis reveals a complex, uneven distribution of inhibition across taxa, suggesting underlying chloroplast microenvironment drivers and providing a new resource for future photosynthesis improvement efforts.

The study examined how polyploidy, hybridization, and incomplete lineage sorting affect phylogenetic reconstructions in the genus Packera, evaluating several published paralog‑processing pipelines. Results showed that the choice of orthology and paralog handling methods markedly altered tree topology, time‑calibrated phylogenies, biogeographic histories, and detection of ancient reticulation, underscoring the need for careful methodological selection alongside comprehensive taxon sampling.

The study introduces a native‑condition method combining cell fractionation and immuno‑isolation to purify autophagic compartments from Arabidopsis, followed by proteomic and lipidomic characterisation of the isolated phagophore membranes. Proteomic profiling identified candidate proteins linked to autophagy, membrane remodeling, vesicular trafficking and lipid metabolism, while lipidomics revealed a predominance of glycerophospholipids, especially phosphatidylcholine and phosphatidylglycerol, defining the unique composition of plant phagophores.

Foliar application of Trichoderma harzianum cell‑free culture filtrates (CF) increased fruit yield, root growth, and photosynthesis in a commercial tomato cultivar under prolonged water deficit in a Mediterranean greenhouse. Integrated physiological, metabolite, and transcriptomic analyses revealed that CF mitigated drought‑induced changes, suppressing about half of water‑stress responsive genes, thereby reducing the plant’s transcriptional sensitivity to water scarcity.

The study used transcriptomic and lipidomic profiling to investigate how chia (Salvia hispanica) leaves respond to short‑term (3 h) and prolonged (27 h) heat stress at 38 °C, revealing rapid activation of calcium‑signaling and heat‑shock pathways and reversible changes in triacylglycerol levels. Nearly all heat‑responsive genes returned to baseline expression after 24 h recovery, highlighting robust thermotolerance mechanisms that could inform improvement of other oilseed crops.

The study characterizes the protein and lipid composition of chloroplast plastoglobules in the B73 maize line during a water-deficit and recovery time course, identifying key polar and neutral lipids and abundant fibrillin proteins. Quantitative proteomics revealed a strong association between Fibrillin 4 and plastoquinone‑9, suggesting a role in redox and prenyl‑lipid metabolism, thereby establishing a foundation for leveraging plastoglobules to enhance crop drought resilience.

The study used Arabidopsis thaliana mutants with low (vtc2, vtc4) and high (vtc2/OE-VTC2) ascorbate levels to examine how ascorbate concentration affects gene expression and cellular homeostasis. Transcriptomic analysis revealed that altered ascorbate levels modulate defense and stress pathways, and that TAA1/TAR2‑mediated auxin biosynthesis is required for coping with elevated ascorbate in a light‑dependent manner.

The study examined how heat stress alters lipid droplet (LD) number and composition in Arabidopsis thaliana roots, revealing degradation of membrane lipids and accumulation of TAGs and LDs. Proteomic and lipidomic analyses of LDs from a specific Arabidopsis mutant identified novel LD-associated proteins, including triterpene biosynthetic enzymes, whose substrates and products also accumulate in LDs, indicating LDs function as both sinks and sources during stress‑induced membrane remodeling and specialized metabolism.

The study demonstrates that knock‑down of CDP‑DAG synthase (CDS) genes in Arabidopsis thaliana enhances resistance to multiple pathogens but incurs a growth penalty, associated with elevated reactive oxygen species and MAPK activation. Integrated lipidomics, transcriptomics, and metabolomics reveal PA accumulation in cds mutants, which triggers JA and SA signaling and increases defense‑related metabolites such as serotonin.

The study examined transcriptomic responses of the tropical seagrass Halophila stipulacea from a pristine and an impacted site under single and combined thermal and excess nutrient stress in mesocosms. Combined stress caused greater gene reprogramming than individual stresses, with thermal effects dominating and the impacted population showing reduced plasticity but higher resilience. Core stress‑response genes were identified as potential early field indicators of environmental stress.