The study functionally characterizes three tomato CNR/FWL proteins (SlFWL2, SlFWL4, SlFWL5) and demonstrates that SlFWL5 localizes to plasmodesmata, where it regulates leaf size and morphology by promoting cell expansion likely through cell‑to‑cell communication. Gain‑ and loss‑of‑function transgenic tomato lines reveal that SlFWL5 is a key regulator of organ growth via modulation of plasmodesmatal signaling.
The study investigates the Arabidopsis ribosomal protein RPS6A and its role in auxin‑related root growth, revealing that rps6a mutants display shortened primary roots, fewer lateral roots, and defective vasculature that are not rescued by exogenous auxin. Cell biological observations and global transcriptome profiling show weakened auxin signaling and reduced levels of PIN auxin transporters in the mutant, indicating a non‑canonical function of the ribosomal subunit in auxin pathways.
The study constructs a ~1‑million‑cell single‑nuclei transcriptome atlas of Arabidopsis leaves to reveal that drought stress accelerates transcriptional programs associated with maturation and aging, thereby limiting leaf growth in proportion to stress intensity. Targeted upregulation of FERRIC REDUCTION OXIDASE 6 in mesophyll cells partially rescues leaf growth under drought, demonstrating the functional relevance of these transcriptional changes.
The study mapped the subcellular localization of isoprenoid biosynthetic enzymes in Marchantia polymorpha, confirming most predictions and identifying oil body cells as primary sites of terpene synthesis. Overexpression and CRISPR knockout of the ABC transporter ABCG1 revealed its essential role in retaining sesquiterpenes within oil bodies, while attempts to boost heterologous diterpene and triterpene production in oil bodies did not increase yields.
Transcriptome responses of two Halophila stipulacea seagrass populations from pristine and impacted habitats, to single and combined thermal and excess nutrient stressors, reveal local adaptive features and core stress-response genes
Authors: Nguyen, H. M., Yaakov, B., Beca-Carretero, P., Procaccini, G., Wang, G., Dassanayake, M., Winters, G., Barak, S.
The study examined transcriptomic responses of the tropical seagrass Halophila stipulacea from a pristine and an impacted site under single and combined thermal and excess nutrient stress in mesocosms. Combined stress caused greater gene reprogramming than individual stresses, with thermal effects dominating and the impacted population showing reduced plasticity but higher resilience. Core stress‑response genes were identified as potential early field indicators of environmental stress.