The study employed a multi‑omics workflow (transcriptomics, ribosome profiling, and proteomics) to uncover small peptides encoded by long non‑coding RNAs (LSEPs) in rice, finding that over 40% of surveyed lncRNAs associate with ribosomes. An optimized small‑peptide extraction followed by LC‑MS/MS identified 403 LSEPs, confirming the peptide‑coding capacity of plant lncRNAs and providing a scalable pipeline for large‑scale screening.
The Global Wheat Full Semantic Organ Segmentation (GWFSS) dataset
Authors: Wang, Z., Zenkl, R., Greche, L., De Solan, B., Bernigaud Samatan, L., Ouahid, S., Visioni, A., Robles-Zazueta, C. A., Pinto, F., Perez-Olivera, I., Reynolds, M. P., Zhu, C., Liu, S., D'argaignon, M.-P., Lopez-Lozano, R., Weiss, M., Marzougui, A., Roth, L., Dandrifosse, S., Carlier, A., Dumont, B., Mercatoris, B., Fernandez, J., Chapman, S., Najafian, K., Stavness, I., Wang, H., Guo, W., Virlet, N., Hawkesford, M., Chen, Z., David, E., Gillet, J., Irfan, K., Comar, A., Hund, A.
The Global Wheat Dataset Consortium released a comprehensive semantic segmentation dataset (GWFSS) of wheat organs across developmental stages, comprising 1,096 fully annotated images and 52,078 unannotated images from 11 institutions. Models based on DeepLabV3Plus and Segformer were trained, with Segformer achieving ≈90% mIoU for leaves and spikes but lower precision (54%) for stems, while also enabling weed exclusion and discrimination of necrotic, senescent, and residue tissues.
RNA‑seq of 328 wheat lines using a pan‑genome reference uncovered over 20,000 additional transcripts beyond the Chinese Spring genome and enabled construction of a pan‑gene eQTL regulatory atlas. Multi‑omics integration identified 231 high‑confidence candidate genes influencing 34 agronomic traits and powdery mildew resistance, with functional validation showing 80% of candidates affecting trait phenotypes via an EMS mutant library.
This review compiles experimental studies on wheat to assess how elevated CO₂, higher temperatures, and water deficit interact and affect productivity and water use. By calculating plasticity indices, the authors find that despite CO₂‑induced gains, overall yield generally declines under combined stress, while water consumption often decreases. They highlight the need for more data to improve and validate crop models under future climate scenarios.
The study introduces Transposase-Accessible Chromosome Conformation Capture (TAC-C), which combines ATAC‑seq and Hi‑C to map fine‑scale chromatin interactions in rice, sorghum, maize, and wheat, revealing genome‑size‑correlated loop structures and distinct C3 vs. C4 patterns. Integration with population genetics shows that loops link distal regulatory elements to phenotypic variation, and SPL transcription factors (TaSPL7/15) modulate photosynthesis‑related genes via these interactions, enhancing photosynthetic efficiency and starch content in wheat mutants.